LOCUS       sequence-118552-         482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-SPR16 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-SPR16
FEATURES             Location/Qualifiers
     source          1..482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..36
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             295..312
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      328..414
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aataacaata acaacaacct cgggatcgaa ggaaggtctt ccggtccaca gcagccttac
       61 ccacaacaac cgtatccgca gcaaccatat ccacagcagc cctaccccca acagccatat
      121 ccccaacaac cataccccca gcagccatac ccgcaacaac cctacccaca gcaaccttat
      181 ccacaacagc cctatcctca acagccttat cctcagcaac cctatccgca acagccgtat
      241 ccccagcaac cgtaccctca acaaccttac cctcaacaac catacggctc gggacatcat
      301 caccaccatc attaataatg aaagcttcaa ataaaacgaa aggctcagtc gaaagactgg
      361 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
      421 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
      481 ta
//
