LOCUS       sequence-118553-         551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-SPR16 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-SPR16
FEATURES             Location/Qualifiers
     source          1..551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(180..270)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(298..325)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(417..503)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(519..536)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
       61 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      181 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      241 atatttgaat gtatttagaa aaataaacaa cggaccgaaa gagtttgtag aaacgcaaaa
      301 aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta tggcgggcgt
      361 cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc cggcggattt
      421 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      481 gactgagcct ttcgttttat ttgaagcttt cattattaat gatggtggtg atgatgtccc
      541 gagccgtatg g
//
