LOCUS       sequence-118556-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses malE-poly-EAK9 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Tang NC, Chilkoti A
  TITLE     Combinatorial codon scrambling enables scalable gene synthesis and 
            amplification of repetitive proteins.
  JOURNAL   Nat Mater. 2016 Jan 4. doi: 10.1038/nmat4521.
  PUBMED    26726995
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2016 Jan 4. doi: 10.1038/nmat4521."
COMMENT     Sequence Label: pMAL-c5X-poly-EAK9
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..32
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             483..500
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      516..602
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acaataacaa caacctcggg atcgaaggaa ggtcttccgg tgcagaggct gaagctaagg
       61 ctaaggctga ggcagaagca aaggcgaaag ctgaagcgga agctaaagca aaagcggaag
      121 cagaagctaa agcgaaagca gaagcagaag ccaaagcaaa ggctgaagcc gaagcgaaag
      181 ctaaggcaga agccgaggca aaagcaaaag cagaagcgga ggcgaaggca aaagctgagg
      241 cggaggctaa agccaaagct gaagcagagg ctaaagctaa agcagaggca gaagctaagg
      301 ccaaggcaga ggccgaggct aaggcaaagg ccgaagcaga agcaaaagct aaagctgaag
      361 ctgaagccaa ggctaaagct gaggctgaag cgaaggctaa agcagaagct gaagctaaag
      421 ctaaggcgga agctgaggcc aaagctaaag ccgaagctga agcaaaagcc aagggctcgg
      481 gacatcatca ccaccatcat taataatgaa agcttcaaat aaaacgaaag gctcagtcga
      541 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      601 atccgccggg agcggatttg aacgttgcga agcaacggcc cggaggg
//
