LOCUS       sequence-118559-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 
            with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS
  TITLE     MicroRNA-based conversion of human fibroblasts into striatal medium 
            spiny neurons.
  JOURNAL   Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102. 
            Epub 2015 Sep 17.
  PUBMED    26379228
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.102"; journalName: "Nat Protoc"; date: 
            "2015-10"; volume: "10"; issue: "10"; pages: "1543-55"
COMMENT     Sequence Label: pmCTIP2-N106
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..450
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(527..547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(945..956)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttcctaggac catggccaag
       61 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
      121 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
      181 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
      241 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
      301 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
      361 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
      421 ctgccctctg gttatgtgtg ggagggctaa cccgggcggg gcgcgtctgg aacaatcaac
      481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
      541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
      601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
      661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
      721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
      781 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
      841 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
      901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
      961 cggaccttcc ttcccgcggc ctgctgcc
//
