LOCUS       sequence-118561-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid. Expresses the reverse 
            tetracycline-controlled transactivator (rtTA) with hygromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS
  TITLE     MicroRNA-based conversion of human fibroblasts into striatal medium 
            spiny neurons.
  JOURNAL   Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102. 
            Epub 2015 Sep 17.
  PUBMED    26379228
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.102"; journalName: "Nat Protoc"; date: 
            "2015-10"; volume: "10"; issue: "10"; pages: "1543-55"
COMMENT     Sequence Label: rtTA-N144
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(26..643,644..649,650..685,686..688,689..724,725..727,
                     728..763,764..772)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(856..879)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 atttcaggtg tcgtgaggat ccaccatgtc tagactggac aagagcaaag tcataaacgg
       61 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
      121 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
      181 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
      241 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
      301 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
      361 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
      421 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
      481 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
      541 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
      601 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct
      661 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
      721 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt aaggatccgc
      781 ggccgcgccg gctctagatc gcgaacgcgt gaattctacc gggtagggga ggcgcttttc
      841 ccaaggcagt ctggagcatg cgctttagca gccccgctgg g
//
