LOCUS       sequence-118564-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector; gateway cassette cloned upstream of basal 
            promoter driving luciferase. Reverse orientation (attR2, attR1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Buckley M, Gjyshi A, Mendoza-Fandino G, Baskin R, Carvalho RS, 
            Carvalho MA, Woods NT, Monteiro AN
  TITLE     Enhancer scanning to locate regulatory regions in genomic loci.
  JOURNAL   Nat Protoc. 2016 Jan;11(1):46-60. doi: 10.1038/nprot.2015.136. Epub 
            2015 Dec 10.
  PUBMED    26658467
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.136"; journalName: "Nat Protoc"; date: 
            "2016-01"; volume: "11"; issue: "1"; pages: "46-60"
COMMENT     Sequence Label: pGL3-LRR
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(126..250)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    289..380
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(321..340)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    394..442
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(573..1028)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(710..731)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     922..941
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
       61 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
      121 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      181 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
      241 acaaagtggt gatgggctag cacgcgtaag agctcggtac ctatcgatag agaaatgttc
      301 tggcacctgc acttgcactg gggacagcct attttgctag tttgttttgt ttcgttttgt
      361 tttgatggag agcgtatgtt agtactatcg attcacacaa aaaaccaaca cacagatgta
      421 atgaaaataa agatatttta ttgcggccgc tccaagtacc tcccgtacct taatattact
      481 tacttatcat ggtagcttgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      541 ccaacagttg cgcagcctga atggcgaatg gcaaattgta agcgttaata ttttgttaaa
      601 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
      661 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
      721 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
      781 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
      841 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
      901 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
      961 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     1021 gggcgcgtca ggtggcactt ttcggg
//
