LOCUS       sequence-118566-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector; gateway cassette cloned upstream of basal 
            promoter driving luciferase. Reverse orientation (attR2, attR1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Buckley M, Gjyshi A, Mendoza-Fandino G, Baskin R, Carvalho RS, 
            Carvalho MA, Woods NT, Monteiro AN
  TITLE     Enhancer scanning to locate regulatory regions in genomic loci.
  JOURNAL   Nat Protoc. 2016 Jan;11(1):46-60. doi: 10.1038/nprot.2015.136. Epub 
            2015 Dec 10.
  PUBMED    26658467
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.136"; journalName: "Nat Protoc"; date: 
            "2016-01"; volume: "11"; issue: "1"; pages: "46-60"
COMMENT     Sequence Label: pGL3-LRR
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        307..503
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      354..489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      533..542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(605..623)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ctcgagatct
      301 gcgatctgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      361 ccctaactcc gcccagttcc gcccattctc cgccccatcg ctgactaatt ttttttattt
      421 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
      481 ttggaggcct aggcttttgc aaaaagcttg gcattccggt actgttggta aagccaccat
      541 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
      601 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
      661 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
      721 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      781 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      841 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
      901 gcagcctacc gtggtgttcg tttccaaaaa
//
