LOCUS       sequence-118568-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a vector that attaches Cerulean(159-238) to the N-terminus 
            of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: Cerulean(159-238) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     291..312
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(404..422)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(405..422)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          506..571
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             701..721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatccg ccaccatggg caagaacggc
      121 atcaaggccc acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      181 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      241 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      301 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagagatct
      361 atcacactgg cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
      421 atgctagagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
      481 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
      541 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
      601 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
      661 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
      721 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
      781 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
      841 caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagat
//
