LOCUS       sequence-118569-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector; gateway cassette cloned upstream of basal 
            promoter driving luciferase. Forward orientation (attR1, attR2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Buckley M, Gjyshi A, Mendoza-Fandino G, Baskin R, Carvalho RS, 
            Carvalho MA, Woods NT, Monteiro AN
  TITLE     Enhancer scanning to locate regulatory regions in genomic loci.
  JOURNAL   Nat Protoc. 2016 Jan;11(1):46-60. doi: 10.1038/nprot.2015.136. Epub 
            2015 Dec 10.
  PUBMED    26658467
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.136"; journalName: "Nat Protoc"; date: 
            "2016-01"; volume: "11"; issue: "1"; pages: "46-60"
COMMENT     Sequence Label: pGL3-LRF
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(160..284)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    323..414
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(355..374)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    428..476
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(744..765)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
       61 cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg gatatgttgt
      181 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      241 atatcatttt acgtttctcg ttcagctttt ttgtacaaac ttgtgatggg ctagcacgcg
      301 taagagctcg gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca
      361 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      421 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      481 ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc
      541 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      601 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      661 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      721 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      781 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      841 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      901 ggagccccc
//
