LOCUS       sequence-118572-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a vector that attaches Cerulean(159-238) to the N-terminus 
            of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH
  TITLE     Live cell analysis of G protein beta5 complex formation, function, 
            and targeting.
  JOURNAL   Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.
  PUBMED    17596375
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2007-10"; volume: "72(4)"; pages: "812-25. Epub 
            2007 Jun 27"
COMMENT     Sequence Label: Cerulean(159-238) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(55..76)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      258..267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(298..317)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(299..317)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(362..565)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(391..411)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtgatagat ctcttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
       61 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
      121 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      181 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagtgggc
      241 cttgatgccg ttcttgccca tggtggcgga tccgagctcg gtaccaagct tgggtctccc
      301 tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga
      361 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      421 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
      481 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
      541 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
      601 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
      661 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
      721 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcg
//
