LOCUS       sequence-118574-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector; gateway cassette cloned upstream of basal 
            promoter driving luciferase. Forward orientation (attR1, attR2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Buckley M, Gjyshi A, Mendoza-Fandino G, Baskin R, Carvalho RS, 
            Carvalho MA, Woods NT, Monteiro AN
  TITLE     Enhancer scanning to locate regulatory regions in genomic loci.
  JOURNAL   Nat Protoc. 2016 Jan;11(1):46-60. doi: 10.1038/nprot.2015.136. Epub 
            2015 Dec 10.
  PUBMED    26658467
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.136"; journalName: "Nat Protoc"; date: 
            "2016-01"; volume: "11"; issue: "1"; pages: "46-60"
COMMENT     Sequence Label: pGL3-LRF
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..139)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        162..358
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      209..344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(460..478)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
       61 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      121 ctttcttgta caaagtggtg atgggctcga gatctgcgat ctgcatctca attagtcagc
      181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      241 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      361 gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa
      421 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
      481 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
      541 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
      601 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
      661 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
      721 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
      781 aaaaaggggt tgcaaa
//
