LOCUS       sequence-118575-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express FLAG-His6-tagged dominant active let60, in which Glycine 12 
            is mutated to Valine, in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y
  TITLE     Characterization of RSF-1, the Caenorhabditis elegans homolog of the
            Ras-association domain family protein 1.
  JOURNAL   Exp Cell Res. 2013 Feb 1;319(3):1-11. doi: 
            10.1016/j.yexcr.2012.10.008. Epub 2012 Oct 26.
  PUBMED    23103556
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2012.10.008"; journalName: "Exp Cell Res"; date: 
            "2013-02-1- 1"; volume: "319"; issue: "3"; pages: "1-11"
COMMENT     Sequence Label: pClneoFH-let60 G12V
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(640..663)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(670..687)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(694..717)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(794..813)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccgggattt tcctccacgt caccgcatgt tagaagactt cctctgccct ctcgaacaga
       61 gagagaccgc acgtcgagtc acattatttg acacttcttc ttcttttgtg gcttattatt
      121 gtcgtgacgc tcacgatgct tgcgaatttc tctaacaagt gtgtaaaatg cttcatcaac
      181 tcccatacgc gttttggcag atgtgtcgac attcggaata ccgtaaccct ttgctgtctc
      241 actgactgtt cggaagtcga ctgatcgaga tgacaaatca catttattcc caaccaagac
      301 cataggaaca tcatctgaat cctttaccct ccgaatctgc tcgcggtagt tagcgacatt
      361 ctcgaaagat ttagcctcgt tgacggcgaa aaccaacaga aatccttcgc ctgtcctcat
      421 gtactgatca cgcatcgccg aatattcttc ttgtccggcg gtatccaata tgtcgaggag
      481 gcatgtctcg ccgtctatca caacctgctt tctgtagctg tcctctatgg tcgggtcgta
      541 ttcttcgaca aagtgattct ggatgagttg aatggtgagt gctgatttac caactcctac
      601 atctccaact accacaagct tgtactccgt catgaattcc ttgtcgtcat cgtctttgta
      661 gtccaattca tgatgatgat gatggtgcaa ttccttgtcg tcatcgtctt tgtagtccat
      721 ggccccggct agcctatagt gagtcgtatt aagtactcta gccttaagag ctgtaattga
      781 actgggagtg gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg
      841 cctatcagaa
//
