LOCUS       sequence-118592-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  4hydroxytamoxifen inducible FlpO recombinase controlled by intestine
            specific promoter (Villin). FlpO is linked to mTQ2 -a blue 
            fluorescent protein. The gene cassette is in a SB transposon 
            context.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Callesen MM, Arnadottir SS, Lyskjaer I, Orntoft MW, Hoyer S, 
            Dagnaes-Hansen F, Liu Y, Li R, Callesen H, Rasmussen MH, Berthelsen 
            MF, Thomsen MK, Schweiger PJ, Jensen KB, Laurberg S, Orntoft TF, 
            Elverlov-Jakobsen JE, Andersen CL
  TITLE     A genetically inducible porcine model of intestinal cancer.
  JOURNAL   Mol Oncol. 2017 Nov;11(11):1616-1629. doi: 10.1002/1878-0261.12136. 
            Epub 2017 Oct 10.
  PUBMED    28881081
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1878-0261.12136"; journalName: "Mol Oncol"; date: 
            "2017-11"; volume: "11"; issue: "11"; pages: "1616-1629"
COMMENT     Sequence Label: Villinpromoter-blue-FlpOERT2
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..353
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     290..311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             369..425
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             438..458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa ctactttagc gacaacgtct atatcaccgc cgacaagcag aagaacggca
      121 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
      181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      241 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
      301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggtatacg
      361 gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg
      421 gacctgctag catggctcct aagaagaaga ggaaggtgat gagccagttc gacatcctgt
      481 gcaagacccc ccccaaggtg ctggtgcggc agttcgtgga gagattcgag aggcccagcg
      541 gcgagaagat cgccagctgt gccgccgagc tgacctacct gtgctggatg atcacccaca
      601 acggcaccgc catcaagagg gccaccttca tgagctacaa caccatcatc agcaacagcc
      661 tgagcttcga catcgtgaac aagagcctgc agttcaagta caagacccag aaggccacca
      721 tcctggaggc cagcctgaag aagctgatcc ccgcctggga gttcaccatc atcccttaca
      781 acggccagaa gcaccagagc gacatcaccg acatcgtgtc cagcctgcag ctgcagttcg
      841 agagcagcga ggaggccg
//
