LOCUS       sequence-118601-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Beck DB, Narendra V, Drury WJ 3rd, Casey R, Jansen PW, Yuan ZF, 
            Garcia BA, Vermeulen M, Bonasio R
  TITLE     In Vivo Proximity Labeling for the Detection of Protein-Protein and 
            Protein-RNA Interactions.
  JOURNAL   J Proteome Res. 2014 Dec 5;13(12):6135-43. doi: 10.1021/pr500196b. 
            Epub 2014 Oct 29.
  PUBMED    25311790
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/pr500196b";
            journalName: "J Proteome Res"; date: "2014-12-5- 5"; volume: "13"; 
            issue: "12"; pages: "6135-43"
COMMENT     Sequence Label: pINTO-NFH::mEzh2
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             183..206
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             216..242
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             249..269
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aaggccacca
      181 tggattacaa ggatgacgac gataagggct ccggataccc atacgatgtg ccagattacg
      241 ctggcagcga gaacctgtac ttccagggct ccggaaccgg tagcggtacc gagctcggat
      301 ccatgggcca gactgggaag aaatctgaga agggaccggt ttgttggcgg aagcgtgtaa
      361 aatcagagta catgagactg agacagctca agaggttcag aagagctgat gaagtaaaga
      421 ctatgtttag ttccaatcgt cagaaaattt tggaaagaac tgaaacctta aaccaagagt
      481 ggaagcagcg gaggatacag cctgtgcaca tcatgacttc tgtgagctca ttgcgcggga
      541 ctagggagtg ttcagtcacc agtgacttgg attttccagc acaagtcatc ccgttaaaga
      601 ccctgaatgc agtcgcctcg gtgcctataa tgtactcttg gtcgccctta caacagaatt
      661 ttatggtgga agacgaaact gttttacata acattcctta tatgggggat gaagttctgg
      721 atcaggatgg cactttcatt gaagaactaa taaaaaatta tgatggaaaa gtgcatggtg
      781 acagagaatg tggatttata aatgatgaaa tttttgtgga gttggtaaat gctcttggtc
      841 aatataatga tgatgatgat gacgatgatg gagatgatcc agatgaaaga gaagaaaaac
      901 agaaagatct agaggataat cgagatgata aagaaacttg cccacctcgg aaatttcctg
      961 ctgataaaat atttgaagcc atttcctcaa tgtttccaga taagggcacc gcagaagaac
     1021 tgaaagaaaa atataaagaa ctc
//
