LOCUS       sequence-11861-e       14279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14279)
  AUTHORS   Losev E, Reinke CA, Jellen J, Strongin DE, Bevis BJ, Glick BS
  TITLE     Golgi maturation visualized in living yeast.
  JOURNAL   Nature. 2006 Jun 22. 441(7096):1002-6.
  PUBMED    16699524
REFERENCE   2  (bases 1 to 14279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jun 22. 441(7096):1002-6."
COMMENT     Sequence Label: YIPlac204-TC-Sec7-6xDsRed-M1
FEATURES             Location/Qualifiers
     source          1..14279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(239..486)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     452..470
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(507..1184)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      1181..1190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1200..1874)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      1871..1880
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1890..2564)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      2561..2570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2580..3254)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      3251..3260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3270..3944)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      3941..3950
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3960..4634)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      4631..4640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10695..11125)
                     /gene="S. cerevisiae TPI1"
                     /label=TPI1 promoter
                     /note="strong constitutive promoter for yeast triose 
                     phosphate isomerase"
     primer_bind     complement(11249..11268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11368..11390
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11509..12183)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(12184..12285)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(12287..12305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12373..12477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12478..12546,12547..13338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12696..12715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13509..14097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13998..14017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14251..14268
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac
      301 atgcgtacac gcgtctgtac agaaaaaaaa gaaaaatttg aaatataaat aacgttctta
      361 atactaacat aactataaaa aaataaatag ggacctagac ttcaggttgt ctaactcctt
      421 ccttttcggt tagagcggat gtggggggag ggcgtgaatg taagcgtgac ataactaatt
      481 acatgatgac tctagagtcg cggccgctac tgggagccgg agtggcgggc ctcggcgtgc
      541 tcgtactgct ccaccacggt gtagtcctcg ttgtggttgg tgatgtccag cttggagtcc
      601 acgtagtggt tgccgggcag ctgcacgggc ttcttggcct tgtacacggt cttgaagtcg
      661 caggtgtagt ggccgccgtc cttcagcttc agggcgtggg agatctcgcc cttcagcacg
      721 ccgtcctggg ggtacagctt ctcggtggag ggctcccagc cggcagtctt cttctgcatt
      781 acggggccgt cggcggggaa gttcacgccc ttgaacttca ccttgtagat gaaggtgccg
      841 tcctgcaggg aggagtcctg ctgcacctcc accacgccgc cgtcctcgaa gttcatggag
      901 cgctcccagg tgaagccctc ggggaaggac agcttcatgt agtcggggat gtcggcgggg
      961 tgcttcacgt aggccttgga gccgtactgg aactgggggg acaggatgtc ccaggcgaag
     1021 ggcagggggc cgcccttggt cacctgcagc ttggcggtct gggtgccctc gtagggcttg
     1081 ccctcgccct cgccctcgat ctcgaagtag tggccgttca cggagccctc catgcgcacc
     1141 ttgaactgca tgaactcctt gatgacgtcc tcggtgttgt ccatggtggc gactagagac
     1201 tgggagccgg agtggcgggc ctcggcgtgc tcgtactgct ccaccacggt gtagtcctcg
     1261 ttgtggttgg tgatgtccag cttggagtcc acgtagtggt tgccgggcag ctgcacgggc
     1321 ttcttggcct tgtacacggt cttgaagtcg caggtgtagt ggccgccgtc cttcagcttc
     1381 agggcgtggg agatctcgcc cttcagcacg ccgtcctggg ggtacagctt ctcggtggag
     1441 ggctcccagc cggcagtctt cttctgcatt acggggccgt cggcggggaa gttcacgccc
     1501 ttgaacttca ccttgtagat gaaggtgccg tcctgcaggg aggagtcctg ctgcacctcc
     1561 accacgccgc cgtcctcgaa gttcatggag cgctcccagg tgaagccctc ggggaaggac
     1621 agcttcatgt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtactgg
     1681 aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt cacctgcagc
     1741 ttggcggtct gggtgccctc gtagggcttg ccctcgccct cgccctcgat ctcgaagtag
     1801 tggccgttca cggagccctc catgcgcacc ttgaactgca tgaactcctt gatgacgtcc
     1861 tcggtgttgt ccatggtggc gactagagac tgggagccgg agtggcgggc ctcggcgtgc
     1921 tcgtactgct ccaccacggt gtagtcctcg ttgtggttgg tgatgtccag cttggagtcc
     1981 acgtagtggt tgccgggcag ctgcacgggc ttcttggcct tgtacacggt cttgaagtcg
     2041 caggtgtagt ggccgccgtc cttcagcttc agggcgtggg agatctcgcc cttcagcacg
     2101 ccgtcctggg ggtacagctt ctcggtggag ggctcccagc cggcagtctt cttctgcatt
     2161 acggggccgt cggcggggaa gttcacgccc ttgaacttca ccttgtagat gaaggtgccg
     2221 tcctgcaggg aggagtcctg ctgcacctcc accacgccgc cgtcctcgaa gttcatggag
     2281 cgctcccagg tgaagccctc ggggaaggac agcttcatgt agtcggggat gtcggcgggg
     2341 tgcttcacgt aggccttgga gccgtactgg aactgggggg acaggatgtc ccaggcgaag
     2401 ggcagggggc cgcccttggt cacctgcagc ttggcggtct gggtgccctc gtagggcttg
     2461 ccctcgccct cgccctcgat ctcgaagtag tggccgttca cggagccctc catgcgcacc
     2521 ttgaactgca tgaactcctt gatgacgtcc tcggtgttgt ccatggtggc gactagagac
     2581 tgggagccgg agtggcgggc ctcggcgtgc tcgtactgct ccaccacggt gtagtcctcg
     2641 ttgtggttgg tgatgtccag cttggagtcc acgtagtggt tgccgggcag ctgcacgggc
     2701 ttcttggcct tgtacacggt cttgaagtcg caggtgtagt ggccgccgtc cttcagcttc
     2761 agggcgtggg agatctcgcc cttcagcacg ccgtcctggg ggtacagctt ctcggtggag
     2821 ggctcccagc cggcagtctt cttctgcatt acggggccgt cggcggggaa gttcacgccc
     2881 ttgaacttca ccttgtagat gaaggtgccg tcctgcaggg aggagtcctg ctgcacctcc
     2941 accacgccgc cgtcctcgaa gttcatggag cgctcccagg tgaagccctc ggggaaggac
     3001 agcttcatgt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtactgg
     3061 aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt cacctgcagc
     3121 ttggcggtct gggtgccctc gtagggcttg ccctcgccct cgccctcgat ctcgaagtag
     3181 tggccgttca cggagccctc catgcgcacc ttgaactgca tgaactcctt gatgacgtcc
     3241 tcggtgttgt ccatggtggc gactagagac tgggagccgg agtggcgggc ctcggcgtgc
     3301 tcgtactgct ccaccacggt gtagtcctcg ttgtggttgg tgatgtccag cttggagtcc
     3361 acgtagtggt tgccgggcag ctgcacgggc ttcttggcct tgtacacggt cttgaagtcg
     3421 caggtgtagt ggccgccgtc cttcagcttc agggcgtggg agatctcgcc cttcagcacg
     3481 ccgtcctggg ggtacagctt ctcggtggag ggctcccagc cggcagtctt cttctgcatt
     3541 acggggccgt cggcggggaa gttcacgccc ttgaacttca ccttgtagat gaaggtgccg
     3601 tcctgcaggg aggagtcctg ctgcacctcc accacgccgc cgtcctcgaa gttcatggag
     3661 cgctcccagg tgaagccctc ggggaaggac agcttcatgt agtcggggat gtcggcgggg
     3721 tgcttcacgt aggccttgga gccgtactgg aactgggggg acaggatgtc ccaggcgaag
     3781 ggcagggggc cgcccttggt cacctgcagc ttggcggtct gggtgccctc gtagggcttg
     3841 ccctcgccct cgccctcgat ctcgaagtag tggccgttca cggagccctc catgcgcacc
     3901 ttgaactgca tgaactcctt gatgacgtcc tcggtgttgt ccatggtggc gactagagac
     3961 tgggagccgg agtggcgggc ctcggcgtgc tcgtactgct ccaccacggt gtagtcctcg
     4021 ttgtggttgg tgatgtccag cttggagtcc acgtagtggt tgccgggcag ctgcacgggc
     4081 ttcttggcct tgtacacggt cttgaagtcg caggtgtagt ggccgccgtc cttcagcttc
     4141 agggcgtggg agatctcgcc cttcagcacg ccgtcctggg ggtacagctt ctcggtggag
     4201 ggctcccagc cggcagtctt cttctgcatt acggggccgt cggcggggaa gttcacgccc
     4261 ttgaacttca ccttgtagat gaaggtgccg tcctgcaggg aggagtcctg ctgcacctcc
     4321 accacgccgc cgtcctcgaa gttcatggag cgctcccagg tgaagccctc ggggaaggac
     4381 agcttcatgt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtactgg
     4441 aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt cacctgcagc
     4501 ttggcggtct gggtgccctc gtagggcttg ccctcgccct cgccctcgat ctcgaagtag
     4561 tggccgttca cggagccctc catgcgcacc ttgaactgca tgaactcctt gatgacgtcc
     4621 tcggtgttgt ccatggtggc gactagtgga tccgtagaaa ggtataattc accaactctg
     4681 cttagaaatt gttttatagc atgtctcaac tccgttggaa cgctcttatc taatatttga
     4741 atcaccagag catacatagc aggacagtat agtctaaagt cttcatcatc aaattcgtag
     4801 taaccttgta atatttcaac tatcactgaa cgccaggcat tgatactacg ttccatggtt
     4861 ctatcatcca aagaaacata accctctacg acctggatgc aaatggtgat cagacgagtt
     4921 attaaatcag ccttcttctc atcatcgttc agatacaact gaaacataat gtcaaggaga
     4981 actgcagcag cactcgtttc ttgtttcaac aggtttggta ttttatcaac tacacgagcc
     5041 tctactagtc ttgttcttaa cccataatct tcattaaagt cacgagaaaa ttcgtacgat
     5101 ttctccaaca atcttgtaat tctaatggct tccttgtaag ggatacagtg ggcaaaatct
     5161 tcgttctcga ataattcgtt gagcagctct atcatcaaaa gctgtaaaac acatttgaca
     5221 acaatagaat tcttgatgtt tattctccta cgtatatctt cgttactttc gtttcgtttc
     5281 atgtgcttag ttttcttggg gatacttaga ctagcgggct ttggattcaa ttcgtccttt
     5341 accgtcggaa cggaattgcc attattaagc ttagtttgcc cattgagtgt ttcaaccatg
     5401 tcaccaccaa cgtcttcgct ggattcttct tgatgcgctc tttcgaccgt ctcactgata
     5461 ttagaatcgt tctcccttct gtcgttgcta tcatcatctg agtgttggct ggtatcatta
     5521 gttgtttggt gatgagatac agatgatttt cttccttgct gtaacggatc ataatcgaac
     5581 aattcattag cagtggttaa atcaaacaat ttatcgaata cgtcacctat ttgattccaa
     5641 tggtactcat tgaatttaga tacgttttgc aatatcaatt gttgcaggca ggatctccca
     5701 attctagcaa tagtgtcatt ttcttgacaa atacaggata ccagcagacc caaaaatcca
     5761 tcgagcattc tgttcaacga ttcaaagtaa tgcgtaaaaa gggcaatcaa gttccttaag
     5821 gcttgaatta acgtggtcga aagccaaaca cttaaatcat catgactatt gaattggttg
     5881 acttcccaat gtttggataa tacaccgaaa ataggaaata gtaacttctt acaaatcttt
     5941 tcccagaaat catcattgaa tttaccacca tatgccacta gggcatcgaa catatagttt
     6001 aatgcacgtg atctaacttc taagtcttca gctgtcatga tcgtatcatt gaagcagaat
     6061 aacataggga accacacatc ctggaatatg tctttcccac gaagtaaagc atcgttatgt
     6121 tttctttctt cttcagtctt attttcattg taaaaacaga tgtctgcgac gttctgagtc
     6181 atctttctta gagactccaa agcatgtaga gatagctttt ggaatctctt atttttggtg
     6241 atttctctga agacaccaac taactcagaa aaggcatctt cttgagagaa tacgttttcg
     6301 aagtgatttg tcacaatatc attgctaacc agcaattgcg tctttaatac aatggattca
     6361 gtgcttgagc gagccgtata ttgtaaggat tcaagaatag gcttccagcc agatttgata
     6421 ctttccgatt ttgtcaatat gaagttccta aaacactcaa taatcatttc ttgaacttca
     6481 gtgttgccac tattttgaac cgtgtattca aaaggtttta aaaaatcatg ttgaaattca
     6541 aaacctgata attcctcaat atctaaaaat ctcatagaca attgacgcag ggaatcgata
     6601 gcgaaaaata ctactgctaa gttagagttt gtagcaatct tgttgaaagc ttttcccata
     6661 acagcccaaa gcggcgtcca ttctagtttg atacgatcca tattgtagta acatacatcg
     6721 accatttttt gcaaggaaaa cattcttggt gtggaagcat tttcagatga ttcaatttct
     6781 tctaaagata cagcggttaa agctttgata aaatcaacga tagcattacc tgataactcg
     6841 gaacttttgg taaatatatt gtccatcaaa acgactaatt cactggagga aatgaattta
     6901 gagatttcgg gtgataaggt ttgattatgg tgtttttctt gagccaattc tgtgggagtt
     6961 gccttcttgc cccaaacatc aaagaaagat gtattattgg atcttgatga ttcgtaagaa
     7021 actctagggt ttgcaacacg agcttgtgca acatctggaa ccgtatctct gtcgatacct
     7081 ttggatatca attgtagtct ttccatttga gacacgacca gcaaaatgtc cttccacgat
     7141 ccctccaagt aatttccttc tgataacgcg acttcaagaa gaattaccat tgcattgaca
     7201 tttttaactt tgatttcttc aaggttttga aggttacaaa attggactaa cgcacctaca
     7261 aaggaggttc tagcatcatt gattctaaaa gtagaagcaa ttttaattga tattttcaag
     7321 ccttctaaac acttattggt tgtgtcaatg tcatcataat ccttaaatgg gggggttaga
     7381 gctgctaaaa aggacatcca tagtgtctcg aaaattgatt taacatgctc aacgtgggaa
     7441 gcagcataat agacgtctgg gcctccttta tttttgttta aattcttaaa gactaattcc
     7501 gttttagatg aaatttcttt tgagacttga ttatatgcct ctcttgtcaa atcacgagaa
     7561 ttaaagagtt gaaagcagat tgctggttgt tggacaagat tggtatcacc tgaaagcatt
     7621 gcctgatgct gttcagaaat taacttgatt tcattgttag caatttcgtt gaacaaacct
     7681 tccaagaagt ctcttggtaa atctcttccg ttgtcaatac cttcgttgtt ttctaaaaac
     7741 tcttgtaaag acattttatt tttgatttgt gacgaatgta aatcagtatt caacatgatc
     7801 aaagaatacg aaagcacata tgcagtatcc gcctttgaaa agactccggg gttttggtcc
     7861 acaaatcttt ccgcaaattt cagcatgaat ctgtcaattt tttgaccttc tccaggcaat
     7921 ctgaaacttt gtaaaaatga ccttaatgcg tcaacaatgg acataccagt gaagtcaaac
     7981 tcatcaacaa atgcgtgcat tatagcgatg ttcttgtcgt ctccttcgcc tagataatcc
     8041 ccaacagcgg ccatgtcgag gccttctgtt tctaataacc acttggcaat ggaaattgga
     8101 gaatcatctt tcaaaaatcc ttttttaata agtactggaa tagctttctt gggtttattg
     8161 ttgaaaatag caatacattc cgataaagca gttttcctta gtttcaaatt ttcaaattgt
     8221 gtggggtcgt ctgcgtcaat gttttgactg ctcaatgatc tgctttcttc gtcttcatat
     8281 gttggtttga agtcatcatt acttgtcata atagaagacc ttacgtcgtt actcaaagat
     8341 gatctagatt cttgacgagc tgaagatgtt gtgtttagta ataccttatt agcagtgtgt
     8401 gtgtttggat ttaaagcttt gtgagcccat gagcttaagg aacgcaaaac tgacacaatg
     8461 caatttagtg acaccatttt gagagcaaaa tcaagtggaa aaagtaaatt gacttgacca
     8521 acatcaggac tagaagacaa gttggaagaa gtcaaaaggg gcaattgtga gaaattgtag
     8581 gtggacaggg attttgatat ttgttcatcg tagtatgatc tttgagtctg agtaatttcc
     8641 accctagtta aagccaatct tgtcaaataa tcaacagtta tttccattac atttggcatt
     8701 ccagggttac aatcataatt caagtaaaat tcaactaaag ttcttgggtc gttacaaatt
     8761 cgttgaataa cacttaaaaa atatctcttt tgttgggagg tggaagtggt taattctgaa
     8821 atggggaagt agatttctgt taaaaaaact ggaatttcct tcacgaaatc tgctctcaaa
     8881 ttagcaatca atagccacat aatttctaaa gtaacctcga aaactggagc tagaggcgag
     8941 gcagcattcc ttgataaaac aagacgtaaa tattgtctaa ttgaatcgat aaagcacaca
     9001 cgctcctttc ctggtagaaa aatgttgtgg gaaaggaata cgtcaatatg atctttgata
     9061 atagagtaaa tgatgtgaag agataaaagc tttgacctga cggcatgtga cctcatgtcg
     9121 agttctgttt ccaaaggttt agcacatatt ttcgccatga ctctaaacac taagaacgca
     9181 tctttaacag ctagatcttg gttagttatg gcgatagaat caggctgttg agcgtccatt
     9241 agtctttcct catcgtcatt caacttatcc atattttcca gagttaaagg agccggcgtt
     9301 tctgcatttt cagaagcctc ggaaagttct atggcggatt gttgttgatg attttttgtc
     9361 gataaggata ctgctgaaga ggtactttgt ttgagatcga ttttatcata caccgaacta
     9421 ataatttgtg tcaaggtcgc ctgtgcaata ccttgattgg atgggttcaa gcaaaagagc
     9481 aaaacgttgt agattgttct gatagccttt agcaaggaag caccgtggca taaagaactt
     9541 gaatcctctt ctaaaatgca actagataaa gctctaacga tttgtagttc cacacggtca
     9601 tcagtgcctt caccttgaaa acagtctgaa atagtatcaa ttgcagcgtc tataattttt
     9661 tgttttggag gcggcgttat tccatcggca gcatcttgtc gttgatcatt agaggctaaa
     9721 gaatcgggtg gattcactaa cagggtctcg tctagcgatc taaaagaaaa caattttgac
     9781 aggcaatcta aagccaaact ttgaactttg gaggatttgg tacgacagct tgcccttagt
     9841 gcttcgaata ctagtattga atccacgtaa tgtgggttat tcgtggtttg cggatcaaat
     9901 tccttaaatt tttgtaatgt cctttcgata gccttctgag cattcgaatt ctttttaatg
     9961 tccttatcat ttaaaattgt ctccaacgta gttttcacta aaattatggt tgagtttgtg
    10021 ttcgaaccat ttgaagatag tgatatgttc ctcgtatggg tagaccttgg ggcagtagaa
    10081 tcgttggtgg gaacactatc ttgatgagcg atactgggtt cgccagcatt ttcctcttct
    10141 tcttcttctt cttcttcttc ttcttcttct tctgacccgc tttcatcacc agaggagctg
    10201 tttgatgtat ttccatcgct ttcatcagat tcttcatctt ctccagagct actctcagta
    10261 ctttcccctg agactgattc agaatcttcc ccgtcttcgg aactggtttc ggaactactg
    10321 ctatccacgt cctcatcatc ctcgtcccca ttgtcttcat cttcatcttc atcttcatct
    10381 tcatcttcat ctccatcttc accttcatcg tcatggcgtt tgtcatttgt atcttccgtt
    10441 tcttcgggcg ttttcattcc gttggtagcg tcgttacttg tttcttttgt ttctgccgta
    10501 ctgccagcgt tttctggccc tttgcatttt tgatcttcac catttgtctc tttagcttct
    10561 tctttaatag cattctgtgg tttcacagac ggattgactg aagaagcagt ctctacattt
    10621 gaagatattt cgccatcccc tttttcagca tttactactg aattctgttc agacatggta
    10681 ccgagctcga attcttttag tttatgtatg tgttttttgt agttatagat ttaagcaaga
    10741 aaagaataca aacaaaaaat tgaaaaagat tgatttagaa ttaaaaagaa aaatatttac
    10801 gtaagaaggg aaaatagtaa atgttgcaag ttcactaaac tcctaaatta tgctgccctt
    10861 tatattccct gttacagcag ccgagccaaa ggtatatagg ctcctttgca ttagcatgcg
    10921 taacaaacca cctgtcagtt tcaaccgagg tggtatccga gagaattgtg tgattgcttt
    10981 aattaatttc ggagaatctc acatgccact gaagattaaa aactggatgc cagaaaaggg
    11041 gtgtccaggt gtaacatcaa tagaggaagc tgaaaagtct tagaacgggt aatcttccac
    11101 caacctgatg ggttcctaga tataatctcg aagggaataa gtagggtgat accgcagaag
    11161 tgtctgaatg tattaaggtc cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    11221 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    11281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
    11341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    11401 catcaccgaa acgcgcgaga cgaaagggcg atcttttatg cttgcttttc aaaaggcttg
    11461 caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct atttcttagc
    11521 atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct ccacacctcc
    11581 gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat tttctggcgt
    11641 cagtccacca gctaacataa aatgtaagct ctcggggctc tcttgccttc caacccagtc
    11701 agaaatcgag ttccaatcca aaagttcacc tgtcccacct gcttctgaat caaacaaggg
    11761 aataaacgaa tgaggtttct gtgaagctgc actgagtagt atgttgcagt cttttggaaa
    11821 tacgagtctt ttaataactg gcaaaccgag gaactcttgg tattcttgcc acgactcatc
    11881 tccatgcagt tggacgatat caatgccgta atcattgacc agagccaaaa catcctcctt
    11941 aggttgatta cgaaacacgc caaccaagta tttcggagtg cctgaactat ttttatatgc
    12001 ttttacaaga cttgaaattt tccttgcaat aaccgggtca attgttctct ttctattggg
    12061 cacacatata atacccagca agtcagcatc ggaatctagt gcacattctg cggcctctgt
    12121 gctctgcaag ccgcaaactt tcaccaatgg accagaacta cctgtgaaat taataacaga
    12181 catactccaa gctgcctttg tgtgcttaat cacgtatact cacgtgctca atagtcacca
    12241 atgccctccc tcttggccct ctccttttct tttttcgacc gaattggcct cgtgatacgc
    12301 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    12361 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    12421 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    12481 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    12541 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    12601 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    12661 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    12721 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    12781 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    12841 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    12901 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    12961 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    13021 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    13081 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    13141 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    13201 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    13261 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    13321 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    13381 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    13441 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    13501 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    13561 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    13621 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    13681 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    13741 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    13801 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    13861 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    13921 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    13981 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    14041 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    14101 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    14161 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    14221 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
