LOCUS       sequence-118613-        7216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector expressing Nrf2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Xiong W, MacColl Garfinkel AE, Li Y, Benowitz LI, Cepko CL
  TITLE     NRF2 promotes neuronal survival in neurodegeneration and acute nerve
            damage.
  JOURNAL   J Clin Invest. 2015 Apr;125(4):1433-45. doi: 10.1172/JCI79735. Epub 
            2015 Mar 23.
  PUBMED    25798616
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI79735"; 
            journalName: "J Clin Invest"; date: "2015-04"; volume: "125"; issue:
            "4"; pages: "1433-45"
COMMENT     Sequence Label: AAV-CMV-Nrf2
FEATURES             Location/Qualifiers
     source          1..7216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     enhancer        212..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     746..766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     792..816
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          929..1404
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     RBS             2249..2257
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(3294..3310)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3295..3311)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      3999..4512
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4086..4105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4296..4317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4994..5013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5113..5135
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5173..5191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5259..5363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5364..5432,5433..6224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5582..5601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6395..6983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6884..6903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtacatttat
      181 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag
      241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      601 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      721 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      781 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
      841 tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt
      901 accgagctcg gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc
      961 cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat
     1021 attgaccaaa tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat
     1081 atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata
     1141 atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg
     1201 gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg
     1261 taagaggttt catattgcta atagcagcta caatccagct accattctgc ttttatttta
     1321 tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc
     1381 atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat
     1441 cactttggca aagaattccg cgggcggccg catgatggac ttggagttgc caccgccagg
     1501 actacagtcc cagcaggaca tggatttgat tgacatcctt tggaggcaag acatagatct
     1561 tggagtaagt cgagaagtgt ttgactttag tcagcgacag aaggactatg agttggaaaa
     1621 acagaaaaaa ctcgaaaagg aaagacaaga gcaactccag aaggaacagg agaaggcctt
     1681 tttcgctcag tttcaactgg atgaagaaac aggagaattc ctcccaattc agccggccca
     1741 gcacatccag acagacacta gtggatccgc cagctactcc caggttgccc acattcccaa
     1801 acaagatgcc ttgtactttg aagactgtat gcagcttttg gcagagacat tcccatttgt
     1861 tgatgaccat gagtcgcttg ccctggatat ccccagccac gctgaaagtt cagtcttcac
     1921 tgcccctcat caggcccagt ccctcaatag ctctctggag gcagccatga ctgatttaag
     1981 cagcatagag caggacatgg agcaagtttg gcaggagcta ttttccattc ccgaattaca
     2041 gtgtcttaat accgaaaaca agcagctggc tgatactacc gctgttccca gcccagaagc
     2101 cacactgaca gaaatggaca gcaattacca tttttactca tcgatctcct cgctggaaaa
     2161 agaagtgggc aactgtggtc cacatttcct tcatggtttt gaggattctt tcagcagcat
     2221 cctctccact gatgatgcca gccagctgac ctccttagac tcaaatccca ccttaaacac
     2281 agattttggc gatgaatttt attctgcttt catagcagag cccagtgacg gtggcagcat
     2341 gccttcctcc gctgccatca gtcagtcact ctctgaactc ctggacggga ctattgaagg
     2401 ctgtgacctg tcactgtgta aagctttcaa cccgaagcac gctgaaggca caatggaatt
     2461 caatgactct gactctggca tttcactgaa cacaagtccc agccgagcgt ccccagagca
     2521 ctccgtggag tcttccattt acggagaccc accgcctggg ttcagtgact cggaaatgga
     2581 ggagctagat agtgcccctg gaagtgtcaa acagaacggc cctaaagcac agccagcaca
     2641 ttctcctgga gacacagtac agcctctgtc accagctcaa gggcacagtg ctcctatgcg
     2701 tgaatcccaa tgtgaaaata caacaaaaaa agaagttccc gtgagtcctg gtcatcaaaa
     2761 agccccattc acaaaagaca aacattcaag ccgcttagag gctcatctca cacgagatga
     2821 gcttagggca aaagctctcc atattccatt ccctgtcgaa aaaatcatta acctccctgt
     2881 tgatgacttc aatgaaatga tgtccaagga gcaattcaat gaagctcagc tcgcattgat
     2941 ccgagatata cgcaggagag gtaagaataa agtcgccgcc cagaactgta ggaaaaggaa
     3001 gctggagaac attgtcgagc tggagcaaga cttgggccac ttaaaagacg agagagaaaa
     3061 actactcaga gaaaagggag aaaacgacag aaacctccat ctactgaaaa ggcggctcag
     3121 caccttgtat cttgaagtct tcagcatgtt acgtgatgag gatggaaagc cttactctcc
     3181 cagtgaatac tctctgcagc aaaccagaga tggcaatgtg ttccttgttc ccaaaagcaa
     3241 gaagccagat acaaagaaaa actaggcggc cgctctagag gatccaagct tatcgatacc
     3301 gtcgacctcg agtgctttat ttgtgaaatt tgtgatacta ttgctttatt tgtaaccatt
     3361 ataagctgca ataaacaagt taacaacaac aattgcattc attttattgt ttcaggttca
     3421 gggggaggtg tgggaggttt tttaaagggg gagggggtac gtagataagt agcatggcgg
     3481 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3541 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3601 ggcctcagtg agcgagcgag cgcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3661 gcccttccca acagttgcgc agcctgaatg gcgaatggcg attctgttgc aatggctggc
     3721 ggtaatattg ttctggatat taccagcaag gccgatagtt tgagttcttc tactcaggca
     3781 agtgatgtta ttactaatca aagaagtatt gcgacaacgg ttaatttgcg tgatggacag
     3841 actcttttac tcggtggcct cactgattat aaaaacactt ctcaggattc tggcgtaccg
     3901 ttcctgtcta aaatcccttt aatcggcctc ctgtttagct cccgctctga ttctaacgag
     3961 gaaagcacgt tatacgtgct cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4441 aacgtttaca atttaaatat ttgcttatac aatcttcctg tttttggggc ttttctgatt
     4501 atcaaccggg gtacatatga ttgacatgct agttttacga ttaccgttca tcgattctct
     4561 tgtttgctcc agactctcag gcaatgacct gatagccttt gtagagacct ctcaaaaata
     4621 gctaccctct ccggcatgaa tttatcagct agaacggttg aatatcatat tgatggtgat
     4681 ttgactgtct ccggcctttc tcacccgttt gaatctttac ctacacatta ctcaggcatt
     4741 gcatttaaaa tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct
     4801 cccgcaaaag tattacaggg tcataatgtt tttggtacaa ccgatttagc tttatgctct
     4861 gaggctttat tgcttaattt tgctaattct ttgccttgcc tgtatgattt attggatgtt
     4921 ggaatgccct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     4981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5101 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5161 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7201 ggccgattca ttaatg
//
