LOCUS       sequence-118615-        8617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector expressing PGC1a gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Xiong W, MacColl Garfinkel AE, Li Y, Benowitz LI, Cepko CL
  TITLE     NRF2 promotes neuronal survival in neurodegeneration and acute nerve
            damage.
  JOURNAL   J Clin Invest. 2015 Apr;125(4):1433-45. doi: 10.1172/JCI79735. Epub 
            2015 Mar 23.
  PUBMED    25798616
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI79735"; 
            journalName: "J Clin Invest"; date: "2015-04"; volume: "125"; issue:
            "4"; pages: "1433-45"
COMMENT     Sequence Label: AAV-CMV-Flag-PGC1a-6His
FEATURES             Location/Qualifiers
     source          1..8617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     primer_bind     complement(192..211)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        313..692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        693..896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     847..867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     893..917
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1010..1485
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1562..1585
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3986..4003
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4104..4498
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      5400..5913
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5487..5506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5697..5718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6394..6413)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6513..6535
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6573..6591)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6659..6763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6764..6832,6833..7624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6982..7001)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7795..8383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8284..8303
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
      301 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      361 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      421 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      481 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      541 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      601 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      661 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      721 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      781 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      841 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
      901 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      961 accgggaccg atccagcctc ccctcgaagc tgatcctgag aacttcaggg tgagtctatg
     1021 ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg
     1081 gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt gtaattttaa
     1141 aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata ctttccctaa
     1201 tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac cattctaaag
     1261 aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat aaatatttct
     1321 gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct acaatccagc
     1381 taccattctg cttttatttt atggttggga taaggctgga ttattctgag tccaagctag
     1441 gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg
     1501 ctggtctgtg tgctggccca tcactttggc aaagaattcc gcgggcccgg gatccaccat
     1561 ggactacaaa gacgatgacg acaaggcggc cgcgatggct tgggacatgt gcagccaaga
     1621 ctctgtatgg agtgacatag agtgtgctgc tctggttggt gaggaccagc ctctttgccc
     1681 agatcttcct gaacttgacc tttctgaact tgatgtgaat gacttggata cagacagctt
     1741 tctgggtgga ttgaagtggt gtagcgacca atcggaaatc atatccaacc agtacaacaa
     1801 tgagcctgcg aacatatttg agaagataga tgaagagaat gaggcaaact tgctagcggt
     1861 cctcacagag acactggaca gtctccccgt ggatgaagac ggattgccct catttgatgc
     1921 actgacagat ggagccgtga ccactgacaa cgaggccagt ccttcctcca tgcctgacgg
     1981 cacccctccc cctcaggagg cagaagagcc gtctctactt aagaagctct tactggcacc
     2041 agccaacact cagctcagct acaatgaatg cagcggtctt agcactcaga accatgcagc
     2101 aaaccacacc cacaggatca gaacaaaccc tgccattgtt aagaccgaga attcatggag
     2161 caataaagcg aagagcattt gtcaacagca aaagccacaa agacgtccct gctcagagct
     2221 tctcaagtat ctgaccacaa acgatgaccc tcctcacacc aaacccacag aaaacaggaa
     2281 cagcagcaga gacaaatgtg cttccaaaaa gaagtcccat acacaaccgc agtcgcaaca
     2341 tgctcaagcc aaaccaacaa ctttatctct tcctctgacc ccagagtcac caaatgaccc
     2401 caagggttcc ccatttgaaa acaagactat tgagcgaacc ttaagtgtgg aactctctgg
     2461 aactgcaggc ctaactcctc ccacaactcc tcctcataaa gccaaccaag ataacccttt
     2521 caaggcttcg ccaaagctga agccctcttg caagaccgtg gtgccaccgc caaccaagag
     2581 ggcccggtac agtgagtgtt ctggtaccaa aggcagccac tccaccaaga aagggcccga
     2641 gcaatctgag ttgtacgcac aactcagcaa gtcctcaggg ctcagccgag gacacgagga
     2701 aaggaagact aaacggccca gtctccggct gtttggtgac catgactact gtcagtcact
     2761 caattccaaa acggatatac tcattaacat atcacaggag ctccaagact ctagacaact
     2821 agacttcaaa gatgcctcct gtgactggca ggggcacatc tgttcttcca cagattcagg
     2881 ccagtgctac ctgagagaga ctttggaggc cagcaagcag gtctctcctt gcagcaccag
     2941 aaaacagctc caagaccagg aaatccgagc ggagctgaac aagcacttcg gtcatccctg
     3001 tcaagctgtg tttgacgaca aatcagacaa gaccagtgaa ctaagggatg gcgacttcag
     3061 taatgaacaa ttctccaaac tacctgtgtt tataaattca ggactagcca tggatggcct
     3121 atttgatgac agtgaagatg aaagtgataa actgagctac ccttgggatg gcacgcagcc
     3181 ctattcattg ttcgatgtgt cgccttcttg ctcttccttt aactctccgt gtcgagactc
     3241 agtgtcacca ccgaaatcct tattttctca aagaccccaa aggatgcgct ctcgttcaag
     3301 atccttttct cgacacaggt cgtgttcccg atcaccatat tccaggtcaa gatcaaggtc
     3361 cccaggcagt agatcctctt caagatcctg ttactactat gaatcaagcc actacagaca
     3421 ccgcacacac cgcaattctc ccttgtatgt gagatcacgt tcaaggtcac cctacagccg
     3481 taggcccagg tacgacagct atgaagccta tgagcacgaa aggctcaaga gggatgaata
     3541 ccgcaaagag cacgagaagc gggagtctga aagggccaaa cagagagaga ggcagaagca
     3601 gaaagcaatt gaagagcgcc gtgtgattta cgttggtaaa atcagacctg acacaacgcg
     3661 gacagaattg agagaccgct ttgaagtttt tggtgaaatt gaggaatgca ccgtaaatct
     3721 gcgggatgat ggagacagct atggtttcat cacctaccgt tacacctgtg acgctttcgc
     3781 tgctcttgag aatggatata ctttacgcag gtcgaacgaa actgacttcg agctgtactt
     3841 ttgtggacgg aagcaatttt tcaagtctaa ctatgcagac ctagatacca actcagacga
     3901 ttttgaccct gcttccacca agagcaagta tgactctctg gattttgata gtttactgaa
     3961 ggaagctcag agaagcttgc gcaggcatca tcatcatcat cattaactcg agggcccaga
     4021 tctaattcac cccaccagtg caggctgcct atcagaaagt ggtggctggt gtggctaatg
     4081 ccctggccca caagtatcac taagctcgct ttcttgctgt ccaatttcta ttaaaggttc
     4141 ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg
     4201 gattctgcct aataaaaaac atttattttc attgcaatga tgtatttaaa ttatttctga
     4261 atattttact aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa gaaatgaaga
     4321 gctagttcaa accttgggaa aatacactat atcttaaact ccatgaaaga aggtgaggct
     4381 gcaaacagct aatgcacatt ggcaacagcc cctgatgcct atgccttatt catccctcag
     4441 aaaaggattc aagtagaggc ttgatttgga ggttaaagtt ttgctatgct gtattttaca
     4501 ttacttattg ttttagctgt cctcatgaat gtcttttcac tacccatttg cttatcctgc
     4561 atctctcagc cttgactcca ctcagttctc ttgcttagag ataccacctt tcccctgaag
     4621 tgttccttcc atgttttacg gcgagatggt ttctcctcgc ctggccactc agccttagtt
     4681 gtctctgttg tcttatagag gtctacttga agaaggaaaa acagggggca tggtttgact
     4741 gtcctgtgag cccttcttcc ctgcctcccc cactcacagt gacccggaat ccctcgacat
     4801 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     4861 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4921 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4981 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5041 gaatggcgaa tggaattcca gacgattgag cgtcaaaatg taggtatttc catgagcgtt
     5101 tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt
     5161 ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg
     5221 gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact
     5281 tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc
     5341 tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta
     5401 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5461 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5521 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5581 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5641 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5701 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5761 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5821 cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct
     5881 gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg
     5941 attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt
     6001 tgtagagacc tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt
     6061 gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt tgaatcttta
     6121 cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct
     6181 tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca
     6241 accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc
     6301 ctgtatgatt tattggatgt tggaattcct gatgcggtat tttctcctta cgcatctgtg
     6361 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6421 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6481 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6541 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     6601 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     6661 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6721 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6781 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6841 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6901 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6961 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7021 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7081 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7141 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7201 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7261 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7321 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7381 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7441 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7501 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7561 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7621 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7681 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7741 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7801 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7861 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7921 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7981 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8041 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8101 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8161 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8221 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8281 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8341 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8401 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8461 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8521 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8581 accgcctctc cccgcgcgtt ggccgattca ttaatgc
//
