LOCUS       sequence-118620-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For sleeping beauty transposition and establishment of an 
            recombinase mediated cassette exchange docking site. To make RMCE in
            cell line instead of flp in cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Callesen MM, Berthelsen MF, Lund S, Fuchtbauer AC, Fuchtbauer EM, 
            Jakobsen JE
  TITLE     Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines
            as an Alternative to the Flp-in System.
  JOURNAL   PLoS One. 2016 Aug 19;11(8):e0161471. doi: 
            10.1371/journal.pone.0161471. eCollection 2016.
  PUBMED    27541869
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-19"; volume: "11(8):e0161471. doi"; pages: " 
            10.1371/journal.pone.0161471. eCollection 2016"
COMMENT     Sequence Label: Transposon-for RMCE_Sleeping
            Beauty_LIR-CAG-Fr...
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    16..63
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             65..769
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
ORIGIN
        1 ttaagccacc atggagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
       61 ttccatggtg ggtgaggata gcgtgctgat caccgagaac atgcacatga aactgtacat
      121 ggagggcacc gtgaacgacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
      181 cgagggcacc cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
      241 cgacatcctg gctaccagct tcatgtacgg cagcaaaacc tttatcaacc acacccaggg
      301 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga ggatcaccac
      361 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccaga acggctgcct
      421 catctacaac gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa
      481 gaaaacactc ggctgggagg ccagcaccga gatgctgtac cccgctgaca gcggcctgag
      541 aggccatagc cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccctcaa
      601 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggct tctacttcgt
      661 ggacaggaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
      721 gatggctgtg gccaggtact gcgacctgcc tagcaaactg gggcacagct ccggactcag
      781 atctcgagct caagcttcga attctgcagt cgacggtacc gcggg
//
