LOCUS       sequence-118621-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RMCE donor plasmid for FlpO. the marker gene is green fluorescent 
            (mWasabi) with PA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Callesen MM, Berthelsen MF, Lund S, Fuchtbauer AC, Fuchtbauer EM, 
            Jakobsen JE
  TITLE     Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines
            as an Alternative to the Flp-in System.
  JOURNAL   PLoS One. 2016 Aug 19;11(8):e0161471. doi: 
            10.1371/journal.pone.0161471. eCollection 2016.
  PUBMED    27541869
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-19"; volume: "11(8):e0161471. doi"; pages: " 
            10.1371/journal.pone.0161471. eCollection 2016"
COMMENT     Sequence Label: Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..41
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             complement(146..850)
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELY"
     regulatory      847..856
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctattccgaa gttcctattc ttcaaatagt ataggaactt ccctgcaggt taattaaagc
       61 aaacgggccc tctagactcg agcggccgcc actgtgctgg atatctgcag aattccacca
      121 cactggacta gtggatccga gctcggtaca gctcgtccat gccgtcggtg gagttgcggg
      181 ccacggcgat ctcgtaaacg gtcaccttgt tgtagtcctt gtcgtggttc aggatctcga
      241 tgcggtggtc cacaaagtga tagtcgggca gcttcaccgc cttcttggcc ctgtagatgg
      301 tcttgaagtc aacgcggtgg tggccgccgc cctccagcag cagcttcatc ttgacgtcgc
      361 ccttcagcac gccgtcgcgc acgtacatcc tctcggtgga ggcgtcccag ccggtggtct
      421 ccttctgcat cacggggccg ttggggggga agttctcgcc cttgaggtgt atctcgtaga
      481 tgaaggagtc ctcctccatg gagatgtcgg acttcacctt cacgatgccc ttgtcctcga
      541 aggtcatggt gcgctcccaa gagtagccct cggggaagga ctgcttgaag tagttgggga
      601 tgtcgtcggg gtacttggtg aaggccctgt tgccgtaact gaacgcggtg gtcagaatgt
      661 cgtaggagaa gggcaggggg gctccctcct tcacctccag gttgatggtg ttggtgccgt
      721 cgtagggctt gccctcgccc tcgccctcga tcacgaaggc gtggccattc acgttgccct
      781 ccatcttcag cttgatcttc atgtcgggct tgattacgcc cattgtggtc tcctcgccct
      841 tgctcaccat ggtggcctcg agcggccgcc actgtgctgg atatctgcag a
//
