LOCUS       sequence-118623-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducbile color change and cancer regulation: FlpO recombinase 
            dependent expression of KrasG12D cMyc and SV40 large T antigen with 
            doxycycline inducible downregulation through tetKRAB system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Callesen MM, Arnadottir SS, Lyskjaer I, Orntoft MW, Hoyer S, 
            Dagnaes-Hansen F, Liu Y, Li R, Callesen H, Rasmussen MH, Berthelsen 
            MF, Thomsen MK, Schweiger PJ, Jensen KB, Laurberg S, Orntoft TF, 
            Elverlov-Jakobsen JE, Andersen CL
  TITLE     A genetically inducible porcine model of intestinal cancer.
  JOURNAL   Mol Oncol. 2017 Nov;11(11):1616-1629. doi: 10.1002/1878-0261.12136. 
            Epub 2017 Oct 10.
  PUBMED    28881081
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1878-0261.12136"; journalName: "Mol Oncol"; date: 
            "2017-11"; volume: "11"; issue: "11"; pages: "1616-1629"
COMMENT     Sequence Label: 
            LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-S
            V40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(157..351)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(454..474)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atccagaggt tgattatcat atgactagtc ccgggaattc gaagcttgag ctcgactagc
       61 tagctagcta ctagctagtc gagatcctta aactgatgat ttgatttcaa atgcagtctc
      121 tgaatcagga tgggtctctt ggtgaatttc tctctccacc agccagggct cttctccctt
      181 ctccaaccgg aggatcacat ctggcttagt aagctgataa cccaaggaaa ccaggttctt
      241 atagttctcc agcatcacat ttctgtacac gatctgctga gcagtgtcca gcagcttcca
      301 ctcctccctg gtgaagtcca caaatacatc cttgaaggtc accagtgtcc gggaccaggc
      361 agttagtgac ttagcatcca tgccctcctt gttcttgatg atacttcctt gagtgacagc
      421 agagtgctga ggagacaaag caccaccgcc gtcgaccttt ctcttctttt ttggcgaccc
      481 actttcacat ttaagttgtt tttctaatcc gcatatgatt agttccaggc cgaaaaggaa
      541 ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtctcagaa gtgggggcat
      601 agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct gttcctccaa
      661 tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt tctccaggga
      721 gaagccttgc tgacacagga acgcgagctg attttcc
//
