LOCUS       sequence-118624-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pancreas specific expression of the FlpO recombinase controlled by 
            the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent 
            protein. The gene cassette is in a SB transposon context.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Transposon-for RMCE_Sleeping 
            Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pdx1-mTQ2-P2A-FlpO-PA
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..190)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(203..259)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(275..526)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(317..338)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     666..685
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aggtggccct cttgatggcg gtgccgttgt gggtgatcat ccagcacagg taggtcagct
       61 cggcggcaca gctggcgatc ttctcgccgc tgggcctctc gaatctctcc acgaactgcc
      121 gcaccagcac cttggggggg gtcttgcaca ggatgtcgaa ctggctcatc accttcctct
      181 tcttcttagg agccatgcta gcaggtccag ggttctcctc cacgtctcca gcctgcttca
      241 gcaggctgaa gttagtagct ccgcttccgt ataccttgta cagctcgtcc atgccgagag
      301 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      361 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
      421 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
      481 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
      541 cgttgtcgct aaagtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      601 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      661 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      721 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
      781 actgcacgcc ccaggacagg gtggtcacga gggtgggcca
//
