LOCUS       sequence-118648-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in 
            light-inducible protein interaction modules for targeting to the 
            plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pCIBN(deltaNLS)-pmGFP
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    128..193
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(292..313)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     641..660
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag
      181 atctgagtcc ggacttgtac actacataat tacacacttt gtctttgact tctttttctt
      241 ctttttaccc ttatacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      301 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      361 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      421 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
      481 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      541 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      601 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      661 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      721 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
      781 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
      841 cttgccgta
//
