LOCUS       sequence-118651-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of His-tagged espB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Karpman D, Bekassy ZD, Sjogren AC, Dubois MS, Karmali MA, 
            Mascarenhas M, Jarvis KG, Gansheroff LJ, O'Brien AD, Arbus GS, Kaper
            JB
  TITLE     Antibodies to intimin and Escherichia coli secreted proteins A and B
            in patients with enterohemorrhagic Escherichia coli infections.
  JOURNAL   Pediatr Nephrol. 2002 Mar;17(3):201-11.
  PUBMED    11956862
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pediatr 
            Nephrol."; date: "2002-03"; volume: "17(3)"; pages: "201-11"
COMMENT     Sequence Label: pCVD469
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..29
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    45..61
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             80..91
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             110..127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatttatttg ctttgtgagc ggataacaat tataatagat tcaattgtga gcggataaca
       61 atttcacaca gaattcatta aagaggagaa attaactatg agaggatcgc atcaccatca
      121 ccatcacgga tccaatacta ttgataatac tcaagtaacg atggttaatt ccgcttcgga
      181 gagtacgacc ggcgcttcca gtgcagttgc cgcatctgct ttatcaattg attcatctct
      241 gcttactgat ggtaaggttg atatttgtaa gctgatgctg gaaattcaaa aactcctcgg
      301 caagatggtg actctattgc aggattacca acaaaaacaa ttggcgcaaa gctatcagat
      361 tcagcaggcc gtttttgaga gccagaataa agctattgag gaaaaaaaag ccgcggcaac
      421 cgctgctttg gttggcggga ttatttcatc agcattgggg atcttaggtt cttttgcagc
      481 aatgaacaac gcggctaaag gggctggtga gattgctgaa aaagcaagct ctgcatcttc
      541 aaaggctgct ggtgcggctt ctgaggttgc aaataaagct ctggtcaagg ctacggaaag
      601 tgttgctgat gtcgcagagg aggcatccag tgcgatgcag aaagcgatgg ccacaacaac
      661 gaaagcagcc agccgtgcat ctggcgttgc agatgatgtt gcgaaagcct ctgactttgc
      721 tgaagatctt gcagacgccg ccgagaagac aagcagaatc aataagttgt tgaattccgt
      781 agataaactg accaatacca cagcatttgt tgccgtgacc agtcttgctg aaggtacgaa
//
