LOCUS       sequence-118654-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDT
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(187..252,253..290)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      454..463
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(505..526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tggagtaggc ggggagaagg ccgcaccctt ctccggaggg gggaggggag tgttgcaata
       61 cctttctggg agttctctgc tgcctcctgg cttctgagga ccgccctggg cctgggagaa
      121 tcccttcccc ctcttccctc gtgatctgca actccagtct tgatatcaaa actctcttaa
      181 ggtagctagg gcgcagtagt ccagggtttc cttgatgatg tcatacttat cctgtccctt
      241 ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg tctttccagt ggggatcgac
      301 ggtatcgata agctgaagtt cctattccga agttcctatt ctatcagaag tataggaact
      361 tcgtagtgcc ccaactgggg taacctttga gttctctcag ttgggggcgt agaattattc
      421 gtatagcata cattatacga agttataccg gtcgccacca tggtgagcaa gggcgaggag
      481 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      541 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      601 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      661 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      721 gccatgcc
//
