LOCUS       sequence-118665-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus
            of protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag C/EBPa
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    119..343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     332..349
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        369..387
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     369..386
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(421..444)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
       61 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
      121 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
      181 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
      241 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
      301 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga
      361 gctctagcat ttaggtgaca ctatagaata gggccctcta gatgcatgct cgagtcatca
      421 cttgtcatcg tcgtccttgt agtcgcctcc cgcgcagttg cccatggcct tgaccaagga
      481 gctctcaggc agctggcgga agatgccccg cagcgtgtcc agttcacggc tcagctgttc
      541 cacccgcttg cgcaggcggt cattgtcact ggtcaactcc agcaccttct gttgcgtctc
      601 cacgttgcgt tgtttggctt tatctcggct cttgcgcacc gcgatgttgt tgcgttcccg
      661 ccgtacccgg tactcgttgc tgttcttgtc caccgacttc ttggctttgc ccgcaccggc
      721 accgctgcca ccgccgccgc ctcccgtgcg gaggtcgggg tgcgcaccgg ccaagccctt
      781 gagcgcgctc ccggggccag gcaggcccgc agcacccaag gcgggcgcag cgtgcgggct
      841 gggcacgggc gtgggcggcg gtgtggggtg gccaggctgc aggtgcatgg tggtctggcc
      901 gcagtgcgcg atctggaact gcaagtgggg
//
