LOCUS       sequence-118667-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the 
            N-terminus of protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag C/EBPb LAP
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             151..216
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg
      121 gccgccagtg tgctggaatt cgccagcatg gactacaaag accatgacgg tgattataaa
      181 gatcatgaca tcgattacaa ggatgacgat gacaaggaag tggccaactt ctactacgag
      241 cccgactgcc tggcctacgg ggccaaggcg gcccgcgccg cgccgcgcgc ccccgccgcc
      301 gagccggcca ttggcgagca cgagcgcgcc atcgacttca gcccctacct ggagccgctc
      361 gcgcccgccg cggacttcgc cgcgcccgcg cccgcccacc acgacttcct ctccgacctc
      421 ttcgccgacg actacggcgc caagccgagc aagaagccgg ccgactacgg ttacgtgagc
      481 ctcggccgcg cgggcgccaa ggccgcgccg cccgcctgct tcccgccgcc gcctcccgcc
      541 gcgctcaagg cggagccagg cttcgaaccc gcggactgc
//
