LOCUS       sequence-118670-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p4947 pcDNA4c hBrd4 full-length
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    115..277
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             290..307
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             311..343
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(347..355,356..370)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttagcgca
      121 gaggcttggg gcagccgagc ggcagccagg ccccggcccg ggcctcggtt ccagaaggga
      181 gaggagcccg ccaaggcgcg caagagagcg ggctgcctcg cagtccgagc cggagaggga
      241 gcgcgagccg cgccggcccc ggacggcctc cgaaaccatg gggggttctc atcatcatca
      301 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga
      361 tgacgataag gtaccaggat ccatgtctgc ggagagcggc cctgggacga gattgagaaa
      421 tctgccagta atgggggatg gactagaaac ttcccaaatg tctacaacac aggcccaggc
      481 ccaaccccag ccagccaacg cagccagcac caaccccccg cccccagaga cctccaaccc
      541 taacaagccc aagaggcaga ccaaccaact gcaatacctg ctcagagtgg tgctcaagac
      601 actatggaaa caccagtttg catggccttt ccagcagcct gtggatgccg tcaagctgaa
      661 cctccctgat tactataaga tcattaaaac gcctatggat atgggaacaa taaagaagcg
      721 cttggaaaac aactattact ggaatgctca ggaatgtatc caggacttca acactatgtt
      781 tacaaattgt tacatctaca acaagcctgg agatgacata gtcttaatgg cagaagctct
      841 ggaaa
//
