LOCUS       sequence-118671-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression plasmid for mouse Myb; adds Flag tag to the N-terminus of
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: pcDNA3 Flag Myb
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc gccagcatgg
      121 actacaaaga cgatgacgac aaggcccgga gaccccgaca cagcatctac agtagcgatg
      181 aagatgatga agacattgag atgtgtgacc atgactacga tgggctgctg cccaaatctg
      241 gaaagcgtca cttggggaaa actaggtgga caagggaaga ggatgagaag ctgaagaagc
      301 tggtggaaca gaacggaaca gacgactgga aagtcattgc caattatctg cccaaccgga
      361 cagatgtgca gtgccaacac cggtggcaga aagtgctgaa ccctgaactc atcaaaggtc
      421 cctggaccaa agaagaagat cagagagtca tagagcttgt ccagaaatat ggtccgaagc
      481 gttggtctgt tattgccaag cacttaaaag ggagaattgg aaagcagtgt cgggagaggt
      541 ggcacaacca tttgaatcca gaagttaaga aaacctcctg gacagaagag gaggacagaa
      601 tcatttacca ggcacacaag cgtctgggga acagatgggc agagatcgca aagctgctgc
      661 ccggacggac tgataatgct atcaagaacc actggaattc caccatgcgt cgcaaggtgg
      721 aacaggaagg ctacctgcag gagccttcca aagccagcca gacgccagtg gccacgagct
      781 tccagaagaa caatcatttg atggggtttg ggcatgcctc acctccatct cagctctctc
      841 caagtggcca gtcctccgtc aacagcgaat atccctatta ccacatcgcc gaagcacaaa
      901 acatctccag tcacgttccc tatcctgtcg cattgcatgt taatatagtc aacgtccctc
      961 agccggctgc ggcagccatc cagagacact ataacgacga agaccctgag aaggaaaagc
     1021 gaataaagga gctggagttg ctcctgatgt c
//
