LOCUS       sequence-118672-       11088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani
            H, Tessier-Lavigne M, Wurst W, Friedel RH
  TITLE     Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar
            development.
  JOURNAL   Mol Cell Neurosci. 2011 Feb;46(2):419-31. doi: 
            10.1016/j.mcn.2010.11.005. Epub 2010 Nov 29.
  PUBMED    21122816
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mcn.2010.11.005"; journalName: "Mol Cell Neurosci"; date:
            "2011-02"; volume: "46"; issue: "2"; pages: "419-31"
COMMENT     Sequence Label: pmPB2-VSV
FEATURES             Location/Qualifiers
     source          1..11088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1095..1127
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             2544..2555
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(6640..6658)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6641..6658)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6678..6695)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6684..6908
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6954..7382
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7041..7060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7251..7272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7391..7411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7396..7726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7577..7712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7793..8587
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7847..7866)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8457..8476
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8761..8882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8798..8817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8852..8871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8931..8947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8931..8947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8944..8966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8955..8971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8979..8985,8986..9003,9004..9009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9024..9045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9162..9179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9333..9921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9413..9432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10092..10883,10884..10952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10715..10734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10953..11057)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcatgc
      901 ctgcaggtca gcttgatttc acccaggcat ctggtccaga gggatccgcc agcccgtcca
      961 gcagcaccat gtgggtgacc aaactcctgc cagccctgct gctgcagcat gtcctcctgc
     1021 atctcctcct gctccccatc gccatcccct atgcagaggg acaaaggaaa agaagaaata
     1081 caattcatga attctataca gacatagaga tgaaccgact tggaaagggc ggagtcgacc
     1141 cggagagttt ccgcagtgag acagagctga accacctggc tgtggatgag gtcacaggtg
     1201 tggtgtatgt cggggcagtg aacgcgctct accagctgag tgccgacctg cacgtccagc
     1261 agcacgtggt cacaggcccc ttcatggata acaagaagtg cacaccaccc attgaggcca
     1321 gccagtgcca cgaagcggtg ctcactgaca acttcaacca gctgctgctg ctcgacccac
     1381 cggggaaacg cctggtggag tgtggcagcc tcttcaaggg catctgtgcc ctgcgcgcca
     1441 tgagcaacat ctcggtgcgc ctcttctacg aggatggcag cggcgagaag tccttcgtgg
     1501 ccagcaatga cgagagagtg gccaccgtgg ggctggtgac ctccacgcgc cccgacggcg
     1561 agcgcgtgct gtttgtgggc aaaggcaatg ggccgcacga caatggtatc atcgtgagca
     1621 cccgcctgct ggacagggct gagggccggg aggcctttga ggcctactct gaccacacca
     1681 ccttcaaggc cggctacctg tccacgaata ctcagcagtt tgtcgcagcc tttgaggatg
     1741 atttctatgt cttcttcgtc tttaaccatc aagacaagca tcctgctaag aaccgcacgc
     1801 tgctggcgcg catgtgcaaa gacgacccct cctactattc ctatgtagag atggacctgc
     1861 agtgtcagga tcccagtgac ccccaagact ctgcctttgg cacctgccta gccgcttctg
     1921 tagccacatc gggtgctggc agagcacttt atgctgtctt cagcagagat ggccggagca
     1981 ctgggggacc tggcgcgggc ctctgtgtgt tcccgctaga taaagtccgt gagaagatag
     2041 aagccaaccg taacgcctgc tacacaggtg cccgagaggc aggccgcact atcttctaca
     2101 agcccttcca cggagaaatc cagtgtggcg gccatctgat aggtgccagc gagagcttcc
     2161 catgcggctc ggagcatctg ccctatccac tgggcagtcg tgatggactc gtcgccacag
     2221 ccgtgctgca ccgcgggggc ctgaatctga cagcagtgac agtaactgcc gagaatgacc
     2281 acactgttgc gttcctgggc acctcagatg gccggatcct taaggtgtac cttgctccag
     2341 acggcacttc tgcagagtat ggttctatcc cagtagacat caataagaaa atcaagcagg
     2401 acctggcact gtctggaaac ctgtccagtc tgtatgctat gacccaggac aaggtgttcc
     2461 ggctcccagt acaagaatgc ctgagctatg taacctgcgc tcagtgtcgt gactcccaag
     2521 atccctactg tggctggtgt gtcatcgagg gacgatgcac caggaagtcc gagtgctcac
     2581 gggccgagga aaccggacac tggctgtgga gccgggagaa gtcctgtgtg gccatcactg
     2641 atgcctttcc acagaacatg agccggcggg cccaaggaga ggtacgcctg tctgtcagcc
     2701 ccctgcccac cctgactgag gacgatgagt tactgtgcct ctttggtgac tcaccacccc
     2761 accctgcccg ggtagaggac gataccgtca tctgtaattc cccaagcagc atccctagta
     2821 caccgccagg ccaagaccat gttgacgtga gcatccagct cctcttaaaa agcggcagtg
     2881 tcttcctcac ctcccaccag tatcccttct atgactgccg tgaggccatg agcctggtgg
     2941 agaacctgcc gtgcatctct tgtgctagca accgctggac ttgccaatgg gacctgcagt
     3001 actacgagtg tcgggaggct tcgcccaacc cagaggaggg aatcatacgt gcccacatgg
     3061 aggacaactg cccccagttc ctggcccctg accctttggt catccccatg aaccatgaga
     3121 cagaggtgac cttccagggc aagaacttgg agactgtgaa ggtttcttcc ctgtatgtgg
     3181 gcagtgagct gctgaatttt gaagagactg tgaccatgca tgaatcagac accttctctt
     3241 ttaggacccc aaagctatcc catgatggta atgagacact gcctcttcac ctgtatgtta
     3301 agtcctttgg caagaacatt gacagcaagc tacaagtgac tctctacaac tgctcctttg
     3361 gccgcagtga ctgtagcctg tgtctggctg ccgatcctgc ctacaggtgt gtgtggtgcc
     3421 gtgggcagaa ccggtgcgtg tacgaagctc tgtgcagcaa tgtcacttct gagtgcccac
     3481 caccagttat cactaggatc cagcctgaga cgggcccgct gggtgggggc atcctggtca
     3541 ctatccatgg gtccaatctg ggtgtcaaag cagatgacgt caagaagata actgtggctg
     3601 gccagaactg tgcctttgaa ccaagagggt actccgtatc cacccggatt gtgtgtgcaa
     3661 ttgaggcttc ggagatgccc ttcacaggag gcattgaggt ggatgttaat ggaaaactcg
     3721 gccattcacc gccacacgtc cagttcactt atcaacaacc ccagcctctc agtgtggagc
     3781 cacgacaggg gccacaggca ggtggcacca cattgaccat caatggcact cacctggaca
     3841 caggctccaa ggaggatgtg cgggtgacac tcaatgacgt cccttgtgaa gtgacaaagt
     3901 ttggagcaca gctacagtgt gtcacaggtc aacagttggc tccaggccag gtgacactag
     3961 aaatctacta tgggggctcc agagtgccca gccccggcat ctctttcacc tactgcgaga
     4021 accccatgat acgagccttt gagccattga gaagctttgt cagtggtggc cggagcatca
     4081 acgttactgg ccagggcttc agcctcatcc agaagtttgc catggttgtc atcgctgagc
     4141 ccttgcggtc ctggaggcgg cggcggcggg aggctggagc cctggagcgt gtgacggtcg
     4201 agggcatgga gtacgtgttc tacaacgaca ccaaggtcgt cttcttgtct cctgctgtcc
     4261 ccgaagagcc cgaggcttac aacctcaccg tgctgatacg gatggacggt cactgtgccc
     4321 cgctcaggac cgaagccgga gtctttgagt atgtggcaga ccccaccttt gagaacttca
     4381 cgggtggcgt caaaaagcag gtcaacaagc tcatccacgc ccggggaacc aatctgaaca
     4441 aagccatgac tctggaggag gcagaggcat ttgtgggtgc tgagcgttgc attatgaaga
     4501 cgctgaccga gacggacctg tactgcgagc ccccagaggt gcagcctcca cccaagcggc
     4561 ggcagaagcg agacacggca cacaacctac ctgagttcat tgtgaagttt ggctcccgag
     4621 agtgggtgct aggccgagtg gagtacgaca cgcgcgcgag tgacgtgccg ctcagtctca
     4681 tcctgcctct ggtcatggta cccatggtgt ttatcatcgt ggtctccatc tactgctact
     4741 ggaggaagag ccagcaggcc gagcgcgagt acgagaagat caaatcccag ctcgagggct
     4801 tggaggagag cgtgcgtgac cgctgcaaga aggaattcac agacctgatg atcgagatgg
     4861 aggaccagac gaatgacgtg catgaggcag gcatccccac gctggactac aagacctaca
     4921 ccgaccgcgt attcttcctg ccatccaagg atggtgacaa ggatgtgatg atcaccggca
     4981 agctagacat ccctgagtca cggcggccca ttgtggagca agccctctac cagttctcca
     5041 acctgcttaa tagcaagtcc ttcctcatca atttcatcca caccctggag aatcagcgtg
     5101 agttctcagc tcgggccaag gtctactttg catcactgct gactgtggcc ctgcacggga
     5161 agctggagta ctacacggac atcatgcgca cgctcttcct ggagctcatg gagcagtatg
     5221 tggtggccaa gaaccccaag ctaatgctgc gcaggtctga gacagtggtg gagaggatgc
     5281 tgtccaactg gatgtccatc tgtctgtacc agtacctcaa ggatagtgca ggggagcctc
     5341 tctacaagct cttcaaggcc atcaaacacc aggtggaaaa ggggcccgtg gatgctgtgc
     5401 agaagaaggc caagtacacc ctcaatgaca caggcctgct cggggacgat gttgagtatg
     5461 cgcctctgac cgtgagcgtg attgttcagg atgaagggat cgatgccatc ccggttaagg
     5521 tcctcaactg tgacaccatc tctcaggtca aagagaagat catcgaccaa gtgtaccgta
     5581 ctcagccctg ctcctgctgg cccaagccag acagtgtggt gcttgaatgg cgtcctgggt
     5641 ccacggccca gattctgtct gacttggacc ttacctccca gcgggagggc cggtggaagc
     5701 gcatcaacac cctgatgcac tacaacgtcc gggatggagc caccctcatc ctgtctaagg
     5761 tgggagtctc ccagcagcca gaagacagcc aacaggactt gccaggggag cgccatgccc
     5821 ttctggaaga ggaaaaccgc gtgtggcact tggtgcggcc aacagatgag gtagatgaag
     5881 gcaagtccaa gcgcggcagc atgaaggaga aagagcggac taaggccatc acagagatct
     5941 acctgacgcg gctactctcc gtcaagggca cactgcagca gtttgtggac aacttcttcc
     6001 aaagcgtgct ggcgcctggg catgcggtgc cacccgccgt caagtacttc ttcgatttct
     6061 tggacgagca ggcagagaag catgacatcc gagatgagga taccatccat atctggaaaa
     6121 ccaacagttt accacttcgg ttctgggtga acatcctgaa gaaccctcat ttcatctttg
     6181 atgtccacgt ccacgaagtg gtggacgcct ccctgtcagt catcgcacag accttcatgg
     6241 acgcctgtac tcgcacggag cacaagctga gccgagactc tcccagcaac aagctgttgt
     6301 atgctaagga gatctctacc tacaagaaga tggtggagga ctactacaag ggcatcagac
     6361 agatggtgca ggtcagcgac caggacatga atacgcactt ggcagagatt tcccgggctc
     6421 acacagactc cctgaacaca ctcgtggccc tacaccagct ctaccaatac acacagaagt
     6481 actatgatga gatcatcaat gctctggagg aggaccctgc agcccaaaag atgcaactgg
     6541 ccttccgcct acagcagatt gctgctgcgc ttgagaataa ggttacagac ctctgaccgt
     6601 caatggcggc cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc
     6661 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6721 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6781 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6841 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6901 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6961 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7021 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7081 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt
     7141 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     7201 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     7261 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     7321 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     7381 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg
     7441 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7561 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7741 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     7801 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     7861 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     7921 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     7981 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     8041 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     8101 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     8161 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     8221 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     8281 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     8341 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     8401 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     8461 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     8521 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     8581 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     8641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8881 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     9541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     9781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
