LOCUS       sequence-118675-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid for mouse Pu.1 expression; containing 
            bicistronic IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: MSCV PIG Pu.1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..654
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(55..74)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     551..571
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
       61 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
      121 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
      181 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
      241 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
      301 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
      361 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
      421 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
      481 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
      541 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
      601 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
      661 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc caattccgcc
      721 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
      781 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      841 tgtcttcttg acgagcatt
//
