LOCUS       sequence-118676-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid for mouse Pu.1 expression; containing 
            bicistronic IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: MSCV PIG Pu.1
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(153..155,156..158,159..872)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(198..219)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(459..478)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     806..827
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
       61 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      121 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca
      181 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      241 tgtctggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      301 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      361 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      421 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      481 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      541 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      601 acgtctatat catggccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc
      661 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      721 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      781 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      841 tcactctcgg catggacgag ctgtacaagt aatgaattaa ttaagaatta tcaagcttat
      901 cgataccgtc gacctgcagc caagcttatc g
//
