LOCUS       sequence-118702-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Voltage sensing domain of Ciona with the 
            G122A/D164A/D186A/R217Q/R229I mutations fused to super ecliptic 
            pHlorin A227D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
  TITLE     Combinatorial mutagenesis of the voltage-sensing domain enables the 
            optical resolution of action potentials firing at 60 Hz by a 
            genetically encoded fluorescent sensor of membrane potential.
  JOURNAL   J Neurosci. 2015 Jan 7;35(1):372-85. doi: 
            10.1523/JNEUROSCI.3008-14.2015.
  PUBMED    25568129
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3008-14"; journalName: "J Neurosci"; date: 
            "2015-01-7- 7"; volume: "35"; issue: "1"; pages: "372-85"
COMMENT     Sequence Label: CC3
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..53
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(137..157)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     752..780
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
       61 gcgggtttaa acgggccctc tagactcgag tcatcatttg tatagttcat ccatgccatg
      121 tgtaatccca tcagctgtta caaactcaag aaggaccatg tggtctctct tttcgttggg
      181 atctttcgaa agagtagaag ttgtaaacag gtaatggttg tctggtaaaa ggacgggccc
      241 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcctc catcttcaat
      301 gttgtgtcta attttgaagt tagctttgat tccattcttt tgtttgtctg ccatgatgta
      361 cacctggtga tcgttatagt tgtattccaa tttgtgtcca agaatgtttc catcttcttt
      421 aaaatcaata ccttttaact cgattctatt aacaagggta tcaccttcaa acttgacttc
      481 agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata gttctttcct gtacataacc
      541 ttcgggcatg gcactcttga aaaagtcatg ccgtttcata tgatctgggt atcttgaaaa
      601 gcattgaaca ccataagtta aagtagtgac aagtgttggc catggaacag gtagttttcc
      661 agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt caccctctcc
      721 actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac
      781 tccagtg
//
