LOCUS       sequence-118706-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-11. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: YFP(159-238)-gamma-11 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     273..294
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(614..632)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(615..632)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtaccg agctcggatc cgccaccatg ggcaagaacg gcatcaaggt gaacttcaag
      121 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      181 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
      241 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
      301 gccgggatca ctctcggcat ggacgagctg tacaagagat ccatgcctgc ccttcacatc
      361 gaagatttgc cagagaagga aaaactgaaa atggaagttg agcagcttcg caaagaagtg
      421 aagttgcaga gacaacaagt gtctaaatgt tctgaagaaa taaagaacta tattgaagaa
      481 cgttctggag aggaccctct agtaaaggga attccagaag acaagaaccc ctttaaagaa
      541 aaaggcagct gtgttatttc ataaagatct atcacactgg cggccgctcg agcatgcatc
      601 tagagggccc tattctatag tgtcacctaa atgctagagg atctttgtga aggaacctta
      661 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
      721 tataaaattt ttaagtg
//
