LOCUS       sequence-118709-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches YFP(1-158) to the C-terminus of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Hu Z, Hu J, Zhang Z, Shen WJ, Yun CC, Berlot CH, Kraemer FB, Azhar S
  TITLE     Regulation of expression and function of scavenger receptor class B,
            type I (SR-BI) by Na+/H+ exchanger regulatory factors (NHERFs).
  JOURNAL   J Biol Chem. 2013 Apr 19;288(16):11416-35. doi: 
            10.1074/jbc.M112.437368. Epub 2013 Mar 12.
  PUBMED    23482569
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.437368"; journalName: "J Biol Chem"; date: 
            "2013-04-19- 19"; volume: "288"; issue: "16"; pages: "11416-35"
COMMENT     Sequence Label: YFP(1-158)-STOP
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(48..506,507..509,510..512))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     187..206
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     446..467
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(543..562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(544..562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(607..810)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(636..656)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctnannngct caacggccgc cagtgtgata gatctttact gcttgtcggc catgatatag
       61 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      121 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      181 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      241 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      301 cacatcaggc cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      361 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      421 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      481 ccggtgaaca gctcctcgcc cttgctcacc atggatccga gctcggtacc aagcttgggt
      541 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
      601 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      661 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      721 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      781 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      841 gtactgccaa gtaggaaagt cccataaggt catgtactgg ggcataatgc caggcgggcc
      901 atttaccgtc attgacgtca atangggggc gtacttggca tatgatacac ttgatgtact
      961 gccaagtggg cagtttaccg taaanactcc acccattgac gtcaatggaa aattccctat
     1021 tggcgttact atgggaanat acgtcattat tgacttcaat gggccggggg tctttggggc
     1081 ggtcaaccan ggngggccan ttnacngtaa gttatgta
//
