LOCUS       sequence-118710-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches YFP(1-158) to the C-terminus of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Hu Z, Hu J, Zhang Z, Shen WJ, Yun CC, Berlot CH, Kraemer FB, Azhar S
  TITLE     Regulation of expression and function of scavenger receptor class B,
            type I (SR-BI) by Na+/H+ exchanger regulatory factors (NHERFs).
  JOURNAL   J Biol Chem. 2013 Apr 19;288(16):11416-35. doi: 
            10.1074/jbc.M112.437368. Epub 2013 Mar 12.
  PUBMED    23482569
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.437368"; journalName: "J Biol Chem"; date: 
            "2013-04-19- 19"; volume: "288"; issue: "16"; pages: "11416-35"
COMMENT     Sequence Label: YFP(1-158)-STOP
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(21..39)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(22..39)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          123..188
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             318..338
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    610..744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(647..666)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     701..720
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atgcatctag agggccctat tctatagtgt cacctaaatg ctagaggatc tttgtgaagg
       61 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
      121 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
      181 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
      241 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
      301 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
      361 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
      421 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
      481 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
      541 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      601 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      721 aatgtatctt atcatgtctg gatcgatccc gccatggtat caacgccata tttctattta
      781 ca
//
