LOCUS       sequence-118712-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-5. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: YFP(159-238)-gamma-5 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(266..287)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(509..528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(510..528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(573..776)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(602..622)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgatagatct ctacaaaaag gaacagactt tctggggcct gaagggattt gtacttgaag
       61 atactccagt cagcagaggg tcatgttgag cattctgcag acagaactgt ttcaaatctg
      121 cagctgcctg ggaaaccttc acccgattga gcccggcctc cagccggagc tgttgaacca
      181 cttttttcat agccgcgacg ctggatgaac cagacatgga tctcttgtac agctcgtcca
      241 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      301 cgttggggtc tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca
      361 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      421 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttgccc atggtggcgg
      481 atccgagctc ggtaccaagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc
      541 cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt
      601 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      661 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
      721 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
      781 gtaatagcga tgactaatac gtagatgtac tgccaagtag g
//
