LOCUS       sequence-118715-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal CFP fragment was fused to Gbeta-1. When 
            co-expressed with an amino-trerminal CFP or YFP fragment fused to a 
            Ggamma subunit, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(159-238)-beta-1 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     263..284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg
       61 agctcggatc cgccaccatg ggcaagaacg gcatcaaggc ccacttcaag atccgccaca
      121 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      181 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      241 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      301 ctctcggcat ggacgagctg tacaagagat ccatcgcaac catgagtgag cttgaccagt
      361 tacggcagga ggccgagcaa cttaagaacc agattcgaga cgccaggaaa gcatgtgcag
      421 atgcaactct ctctcagatc acaaacaaca tcgacccagt gggaagaatc caaatgcgca
      481 cgaggaggac actgcggggg cacctggcca agatttacgc catgcactgg ggcacagact
      541 ccaggcttct cgtcagtgcc tcgcaggatg gtaaacttat catctgggac agctacacca
      601 ccaacaaggt ccacgccatc cctctgcgct cctcctgggt catgacctgt gcatatgccc
      661 cttctgggaa ctatgtggcc tgcggtggcc tggataacat ttgctccatt tacaatctga
      721 aaac
//
