LOCUS       sequence-118718-        1462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal 
            expression in C. Elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Nguyen JP, Shipley FB, Linder AN, Plummer GS, Liu M, Setru SU, 
            Shaevitz JW, Leifer AM
  TITLE     Whole-brain calcium imaging with cellular resolution in freely 
            behaving Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Dec 28. pii: 201507110.
  PUBMED    26712014
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Dec 28. pii: 201507110."
COMMENT     Sequence Label: Prab-3::NLS::GCaMP6s
FEATURES             Location/Qualifiers
     source          1..1462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(28..36,37..54,55..57,58..90,91..93,94..117,118..144,
                     145..201,202..207,208..477,478..495,496..930,931..936,
                     937..1380)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      124..133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     414..435
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(541..562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(802..821)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggctccaa agaagaagcg taaggtaatg ggttctcatc atcatcatca tcatggtatg
       61 gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat
      121 ctcgccacca tggtcgactc atcacgtcgt aagtggaata agacaggtca cgcagtcaga
      181 gctataggtc ggctgagctc actcgagaac gtctatatca aggccgacaa gcagaagaac
      241 ggcatcaagg cgaacttcca catccgccac aacatcgagg acggcggcgt gcagctcgcc
      301 taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      361 tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc
      421 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc
      481 ggtaccggag ggagcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      541 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag
      601 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      661 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      721 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag
      781 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      841 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      901 aacatcctgg ggcacaagct ggagtacaac ctgccggacc aactgactga agagcagatc
      961 gcagaattta aagaggcttt ctccctattt gacaaggacg gggatgggac aataacaacc
     1021 aaggagctgg ggacggtgat gcggtctctg gggcagaacc ccacagaagc agagctgcag
     1081 gacatgatca atgaagtaga tgccgacggt gacggcacaa tcgacttccc tgagttcctg
     1141 acaatgatgg caagaaaaat gaaatacagg gacacggaag aagaaattag agaagcgttc
     1201 ggtgtgtttg ataaggatgg caatggctac atcagtgcag cagagcttcg ccacgtgatg
     1261 acaaaccttg gagagaagtt aacagatgaa gaggttgatg aaatgatcag ggaagcagac
     1321 atcgatgggg atggtcaggt aaactacgaa gagtttgtac aaatgatgac agcgaagtga
     1381 gggcgccatg agccgtagac gaaaagcgaa tccgacaaaa ctgagtgaaa acgcgaagaa
     1441 gcttgccaag gaagttgaaa at
//
