LOCUS       sequence-118728-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal CFP fragment was fused to Ggamma-5. When 
            co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(1-158)-gamma-5 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(164..622,623..625,626..628))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     303..322
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     562..583
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      625..634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(665..684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(666..684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcagcagagg gtcatgttga gcattctgca gacagaactg tttcaaatct gcagctgcct
       61 gggaaacctt cacccgattg agcccggcct ccagccggag ctgttgaacc acttttttca
      121 tagccgcgac gctggatgaa ccagacatgg atctctgctt gtcggcggtg atatagacgt
      181 tgtggctgat gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      241 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      301 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      361 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
      421 gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
      481 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
      541 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
      601 tgaacagctc ctcgcccttg ctcaccatgg tggcggatcc gagctcggta ccaagcttgg
      661 gtctccctat agtgagtcgt attaattt
//
