LOCUS       sequence-118730-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal CFP fragment was fused to Ggamma-7. When 
            co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(1-158)-gamma-7 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(235..693,694..696,697..699))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     374..393
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     633..654
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(736..755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(737..755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgtgatagat ctttataaaa taatacaagg tttcttgtcc ttaaaggggt tctccgaggc
       61 agggactccg accagcaggg ggtcgttccg ggcatgttgc tcacagtagc tcatgaggtc
      121 agacgccgct ttggagacct tgatgcgctc aatcccggct tctatgcgta gctgttccac
      181 cagcttccgg gcctgggcta tgttgttagt ggctgacatg gatctctgct tgtcggcggt
      241 gatatagacg ttgtggctga tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      301 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      361 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      421 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      481 gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      541 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      601 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      661 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcggatc cgagctcggt
      721 accaagcttg ggtctcccta tagtgagtcg tattaatttc gataagccag taagcag
//
