LOCUS       sequence-118742-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Shigetomi E, Kracun S, Khakh BS.
  TITLE     Monitoring astrocyte calcium microdomains with improved membrane 
            targeted GCaMP reporters.
  JOURNAL   Neuron Glia Biol. 2010 Dec 16:1-9. [Epub ahead of print]
  PUBMED    21205365
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron Glia
            Biol. 2010 Dec 16:1-9. [Epub ahead of print]"
COMMENT     Sequence Label: pN1-Lck-GCaMP3
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             198..215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             219..251
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(255..263,264..278)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      285..294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             306..362
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     575..596
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(702..723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(963..982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctgccaccat gggctgtggc tgcagctcaa accctgaaga tgactggatg gagaacattg
      121 acgtgtgtga gaactgccat tatcccccgc cgaagcttcg aattgatcca ccggatctcg
      181 ccaccatgcg gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
      241 agcaaatggg tcgggatctg tacgacgatg acgataagga tctcgccacc atggtcgact
      301 catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt cggctgagct
      361 cactcgagaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag gcgaacttca
      421 agatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca
      481 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc gtccagtcca
      541 aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      601 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatgg
      661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctacggca
      781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      841 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      901 acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca
      961 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgaggg
//
