LOCUS       sequence-118743-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Shigetomi E, Kracun S, Khakh BS.
  TITLE     Monitoring astrocyte calcium microdomains with improved membrane 
            targeted GCaMP reporters.
  JOURNAL   Neuron Glia Biol. 2010 Dec 16:1-9. [Epub ahead of print]
  PUBMED    21205365
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron Glia
            Biol. 2010 Dec 16:1-9. [Epub ahead of print]"
COMMENT     Sequence Label: pN1-Lck-GCaMP3
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(145..588)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     704..723
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctcactt cgctgtcatc atttgtacaa actcttcgta
      181 gtttacctga ccatccccat cgatgtctgc ttccctgatc atttcatcaa cctcttcatc
      241 tgttaacttc tctccaaggt ttgtcatcac gtggcgaagc tctgctgcac tgatgtagcc
      301 attgccatcc ttatcaaaca cacggaacgc ttctctaatt tcttcttcac tgtctgtgtc
      361 tttcattttt cttgccatca ttgtcaggaa ctcagggaag tcgattgtgc catcaccgtc
      421 ggcatctact tcattgatca tgtcctgcag ctctgcttct gtggggttct gccccagaga
      481 ccgcatcacc gtccccagct ccttggttgt tattgtccca tccccgtcct tgtcaaatag
      541 ggagaaagcc tctttaaatt ctgcgatctg ctcttcagtc agttggtcac gcgtgttgta
      601 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      661 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      721 gtccttgaag aagatggtgc gctcctggat gtagccttcg ggcatggcgg acttgaagaa
      781 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
      841 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      901 cagcttgccg taggtggcat cgccctcacc ctcgccgga
//
