LOCUS       sequence-118752-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TRABID NZF1-3 (aa 1-178) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINK-TRABID (NZF1-3, aa 1-178)
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(591..614)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(653..675)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgtttaaat ggtctagaaa gctttaatta
       61 ggtctgggat gatcacaaac aacacatctt ttagccttgg cccagttttc atatgtgcaa
      121 acagagcaag tccagtgctg tgtcctagtg ttcagtttat ttctatcatt gtattcctca
      181 caaggatcaa cagaaaaagc aactggtctt gagccagatc ctgaggactg aggagattct
      241 gtaggactcc tggtcctacg ttgggataag cactgggtac atctgattgc tcttggccag
      301 ttcaaatatg tacacatgtg gcatgaccac ttatttgcat tttccatgct atacgaagat
      361 ttcacccttg gtcttgcact agagtctgga catatcaaag gactacttcc tccttcggtg
      421 ctggaaggat cccaatctct accaacatca cttgaaccac ttttaaatgg atcttctgta
      481 ataattgttc cactaggtct ttgggcacga cacatagtac acttgattgc agatggccag
      541 ttttcatacg tacaatattc acaagcccac ttaattccac gttctgacat cgggccctga
      601 aacagaactt ccagaccgct gctcagatcc gattttggag gatggtcgcc accaccaaac
      661 gtggcttgcc agccctgcaa aggccatgct atatacttgc tggatttcaa gtacttatca
      721 atttgt
//
