LOCUS       sequence-118754-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal mCerulean fragment was fused to Ggamma-1. When 
            co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: Cer(1-158)-gamma-1 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     385..404
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     644..665
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      707..716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(747..766)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(748..766)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatctttatg aaatcacaca gcctcctttg agctccttga agggattttt gtcctctggg
       61 atacccttta ctaatggatc ttccccggat ctttcttcaa cataatccct gaattcttca
      121 caacatttgg acaccagcat tctttccagc gtcacttctt tcttgagctg gtcgacttcc
      181 atcttcaatt tgtccttttc tgtcaggtcc tcaatattga tcactggcat ggatctctgc
      241 ttgtcggcgg tgatatagac gttgtcgctg atggcgttgt actccagctt gtgccccagg
      301 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      361 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
      421 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      481 tcggggtagc gggcgaagca ctgcacgccc caggtcaggg tggtcacgag ggtgggccag
      541 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      601 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      661 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcggat
      721 ccgagctcgg taccaagctt gggtctccct atagtgagtc gtattaattt cgataagcca
      781 gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct ccc
//
