LOCUS       sequence-118758-        8924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized GFP for pan-neuronal expression in C. Elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Nguyen JP, Shipley FB, Linder AN, Plummer GS, Liu M, Setru SU, 
            Shaevitz JW, Leifer AM
  TITLE     Whole-brain calcium imaging with cellular resolution in freely 
            behaving Caenorhabditis elegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Dec 28. pii: 201507110.
  PUBMED    26712014
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 Dec 28. pii: 201507110."
COMMENT     Sequence Label: Prab-3::NLS::GFP
FEATURES             Location/Qualifiers
     source          1..8924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4517..4537
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4562..4582
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4625..4653)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5401..5421
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6364..6386)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6486..6505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6583..6601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6669..6773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6774..6842,6843..7634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6992..7011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7805..8393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8294..8313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8547..8564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8681..8702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8717..8722,8723..8740,8741..8747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8755..8771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8760..8782
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8779..8795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8779..8795
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggc gagttttgac tggctttcaa aaactgagtg attactaatt
       61 atttttgaaa aaattaacag ttgtgatatg atatttagac atttttgcaa tatataattt
      121 gtttgaaaaa aaaacatttt ttaatggtct tcgaagctat aatagttttt cgtaaaattt
      181 taaataaaac agctgacaga tatccacatt caataattga aagtatgcag ttctcttttt
      241 ttttgtcaaa tagaaaaaaa gtaacagatg gtcagatata atctaggtta atctgactct
      301 ggtcatcgat gaactcattt tcatcataat cattcttctt tcaaatggtc tgttcggaat
      361 tcgaatattc aataaatgga tatcttatta gatgctccgg atttattcca ccaaacttcc
      421 aagacgtaag ttcaatttaa acaaaattac tatagcttga aaaaatgaga aaaaacgagc
      481 agataaaaaa ataacagaaa aaatccataa aaatgacgag ggttccgtgc gaaaatcgtc
      541 attgtttttg gcttgcatca cacttttccg cgagtcagcc acactgtttt atcgattatt
      601 atttttctga tttcagaaac cggaaactta caactgagat gctttcattt tgattttttt
      661 aaatttagtt ttgatttcag cgatttgttt gaatataatt atgcatgagt tggcttttgc
      721 atttttagat aaataagttc acatgctcgg acaaattgtg tggtttaatc tgtgaaagtt
      781 ttgaatcttc tgtttgaaaa aaaaacattt ttaatttgta tatacgagtg aattggttag
      841 gccctggtta cgttttccag cgaattcatt caaatttctt attttttcta ttttttcaat
      901 taaataattt agttctctat cacatgtgta tgatcaaaat ttcattccta atcgtaagca
      961 aatttaggct catccgtagt ataattttgt tatgaagggc ttcacagtaa aagctcaaac
     1021 tccaacattt agcaactagc aaccaacaaa aaaccaaaga aatgtcagtt ttgaaaagtc
     1081 gttatttctt tgtcttctca caattcacac atcatcttct cgttttcctc cgtttttttg
     1141 tcgtgcttcc ttcttttctt tacacatttt tctaaccttt ttttaagtct atcctctcct
     1201 aatcctttgt actctctaat cagttttgct ggttgtgctg cacaattttt atcagattta
     1261 tgcgcgaaac aaaagcctct cgcggagagc tccgccaaat ccggtcttct tttttccttg
     1321 cctatcggat ttcacaaaca ttcagtttgt cacttgacga tgtgttggag gcattactac
     1381 taggaagtgt tattcatatt ttttattcgt ttctttttgg tgactaaacg aaatattccg
     1441 aacgttttcc tgatttcagg cggcggaaac gaagaagacc atgaataatc aacaggctgc
     1501 cattgcatcc gccagaagtc gagtgagttc tttgaaaatc tttattagag ttatttgtga
     1561 caatttgcca cttttttttt aatgtttaat gaatctgtaa cgaaattaat accaagggtg
     1621 tccaacagaa ctatctgagc ggttatttaa tttttagata gtatatttct tccgatcgta
     1681 acatacatat tttcaagcat agtactgtga tttaagttac agttttccaa tcagtcttga
     1741 ttttaataaa aacttttcaa ctgcaaaaat gcctctgaag tatttttcta tatttttata
     1801 gttgaataat tttttgattg gcacaaagac cgccgagata tgagccgtta aagtaaagct
     1861 gcatggattc aatatggttt gtatttgatt ggagtcactt acatggaaat atttgtattt
     1921 aaatctcata ttttaaaaca agacaaacac caaattaaat ttttattaat ctgaacattt
     1981 ttcaatcgat ttggtaaaca aattattaga tgtttccctc aaaaattgta acaattccta
     2041 aattgtggca cttataataa catcgtcctg aattctcaaa aaaaaccgaa tgctaagaga
     2101 gatttcgttt aactgggacc tgtaactgaa ttttcaaata ttggcagttt ttgataaact
     2161 gggagggaaa tcaaaaacca aaactacagt acccctcgtg ttataataaa ttgtcattca
     2221 ctcctttttt gttcccaaca tttgctccca tctcctgacg agaaagaaca attttatgag
     2281 cacgggaacc cgtgggcgtc catccgttcc tcctgtgcaa tttcagcccc cttcctctac
     2341 attctctgtg ttcccgtttt tatcgtttta tgggtatcgg gaacggacag atcgcttcca
     2401 tttgcttcta ttccgtcctc acaagacgtc ccactaactg attttcttct gatttctttt
     2461 cactgaaaat gggaaagaaa gaaaaagacg atgaatcgac gacttcttct caggagaagg
     2521 tgaagaagaa ggataaggag aaagttcatc taaggctgaa agataaagta aaaaaactta
     2581 gaaaatctaa gtcgattgca gtatcttctc cagctgatta tatgacttgt cagtgtgaac
     2641 catgcaaaaa agtagctctc gaggtaataa tttttaaact caatacgtag ttaagtttga
     2701 actcctttta atttacttat catctaattt accgtcacaa tagtacttcc attagcaatt
     2761 attttaaaat tacatgcata gcttcaatgt tcgttttttt gcgttctcat atcgtatgtt
     2821 gtctgcctct cgaatactga ctgtccgact agaatgtccc ggtaggatta ctgtacggtc
     2881 actgggaata aactgtttct gaaatcaatc tgaaattccg tagatcttct tggttcaatt
     2941 tcttattcct caatctttgg acaacgtcac cttcagagtc gtcgttgagt atcccatcat
     3001 taactagtca atctttctct ctttttcatt tcccccaact tttctgcata ttttttgacg
     3061 acgacgacct cgacggcaac gagaatacat ttatctattt tttagtatag catatataca
     3121 ttttctactt ttctctaacc catttcagac gtatagattt tggttttcga atgcaacttt
     3181 gagatttttc aaagatcttc agatgggagc agtggactgt ctatttgata ttcttaaaat
     3241 gtactctata aaagatgaaa ctgtaatgac caattcccta cataaatatt tttaactaat
     3301 tggtcggata tttggggatc agagaagtac ttgtttttgc gatattaata atgatatatt
     3361 tttctagtaa aaaaatgtta agcgatttgt gaataattaa gttggcttga aaaagatttt
     3421 ttgatagcta tgtattttgt taatttaaat cagattttaa aactttatta ttgactattt
     3481 atatatatta tatattatat attttcatgg tttaatcaaa atctattaaa ttatttctaa
     3541 aatctctaaa tatcgaattc tctaaaaagc ttcatcaaca aaagcatttc aattatctaa
     3601 cacaatgaga ataattcaag aactgaaata gaaagaaatc tagaaaaaaa agagagtgag
     3661 gtgccctatt aaatcggtgc tctcctctct aacagaagcg cctacacgca cccaagagag
     3721 ctctttttct ttcttttttc ggagcacaca tacacgcata cactcctcag aagtgtatag
     3781 agaaaatggg agaagaagcg aaagaagacg aagaagcggt tgtgatgtat gcatcgggcg
     3841 tttctatctc tctccgtgag caacgagcta gtcaacccat cgatccattt ttgtgagcac
     3901 acacagagag agactcaaac acactcacag cttcacactg agctccaaga gctctgtaga
     3961 gagaagccag agtatgcttt cctcgatttt cctaacgtct tcttcttctg cttttttctt
     4021 tttcccaacg agttgtagtc cactaacttc aatttttcct ccagagaaag agttttagag
     4081 agacagctga taaatacact agagaaataa aaagaatgat aaaaatttgc tattttaatc
     4141 agttccctct cgtttctcta agttttgttg ttgggttggg aaattaaaat tggaaaatgg
     4201 aaataacgtt tttttctgtc taatcttgaa ttctccagac atttcttatt tccttttttg
     4261 ttgcaagttt ttcttccttc tctaattttt aatcaaagat atctcgctat gaactttgaa
     4321 tttttgatat tttgattcta taaatacata aagtaatcta aatgctcttt taaaataaat
     4381 ctacagtagc cctattttca gggatccccg ggattggcca aaggacccaa aggtatgttt
     4441 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggaggg taccgagctc
     4501 agaaaaaatg accgctccaa agaagaaacg caaagtaccg agctcagaaa aaatgacggc
     4561 acctaaaaag aaacgcaaag taccggtaga aaaaatgagt aaaggagaag aacttttcac
     4621 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     4681 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     4741 tactggaaaa ctacctgttc catgggtaag tttaaacata tatatactaa ctaaccctga
     4801 ttatttaaat tttcagccaa cacttgtcac tactttctgt tatggtgttc aatgcttctc
     4861 gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     4921 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtaagtttaa
     4981 acagttcggt actaactaac catacatatt taaattttca ggtgctgaag tcaagtttga
     5041 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     5101 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     5161 caaacaaaag aatggaatca aagttgtaag tttaaacatg attttactaa ctaactaatc
     5221 tgatttaaat tttcagaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     5281 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     5341 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     5401 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     5461 agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca
     5521 tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc
     5581 tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt
     5641 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     5701 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     5761 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     5821 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     5881 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     5941 ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct
     6001 catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa
     6061 ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac
     6121 ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga
     6181 aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta
     6241 gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact
     6301 gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     6361 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6421 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     6481 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6541 cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat
     6601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8641 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8701 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8761 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt
     8821 aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
     8881 aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
