LOCUS       sequence-118760-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal mCerulean fragment was fused to Ggamma-2. When 
            co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: Cer(1-158)-gamma-2 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     379..398
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     638..659
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      701..710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(741..760)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(742..760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atagatcttt aaaggatagc acagaaaaac ttcttctccc taaatgggtt ttctgaagcc
       61 ggaacaggtg tcaggagggg atcttccttg gcatgcgctt cacagtaggc catcaaatct
      121 gcagctgcct tggacacctt tatcctatcg atgttggctt ccatcttcag ctgttctacc
      181 agtttcctgg cttgtgctat gctggcggtg ttgttgctgg ccatggatct ctgcttgtcg
      241 gcggtgatat agacgttgtc gctgatggcg ttgtactcca gcttgtgccc caggatgttg
      301 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      361 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      421 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      481 tagcgggcga agcactgcac gccccaggtc agggtggtca cgagggtggg ccagggcacg
      541 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      601 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      661 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc ggatccgagc
      721 tcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa g
//
