LOCUS       sequence-118763-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An amino-terminal mCerulean fragment was fused to Ggamma-5. When 
            co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: Cer(1-158)-gamma-5 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     370..389
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     629..650
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(732..751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(733..751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atagatctct acaaaaagga acagactttc tggggcctga agggatttgt acttgaagat
       61 actccagtca gcagagggtc atgttgagca ttctgcagac agaactgttt caaatctgca
      121 gctgcctggg aaaccttcac ccgattgagc ccggcctcca gccggagctg ttgaaccact
      181 tttttcatag ccgcgacgct ggatgaacca gacatggatc tctgcttgtc ggcggtgata
      241 tagacgttgt cgctgatggc gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      301 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      361 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      421 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
      481 aagcactgca cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      541 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      601 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      661 accccggtga acagctcctc gcccttgctc accatggtgg cggatccgag ctcggtacca
      721 agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag cagtgggttc
      781 tctagttagc cagagagctc tgcttatata gacctcccac cg
//
