LOCUS       sequence-118768-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches CFP(1-158) to the C-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  TITLE     Unpublished BiFC Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CFP(1-158)Stop in pcDNA1/amp
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(104..106,107..109,110..568)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(149..170)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(410..429)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(630..648)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(631..648)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          732..797
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ttagcganct ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccatggtga gcaagggcga
      121 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      181 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      241 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      301 ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
      361 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      421 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      481 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      541 catcagccac aacgtctata tcaccgccga caagcagtaa agatctatca cactggcggc
      601 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagaggatct
      661 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
      721 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
      781 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
      841 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
      901 ctgctgactc tcaacattct actcctccna aaaaggaaga gaaaggtaga agaccccaag
      961 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     1021 gcttgctttg ctatttacac cacaangnaa aagctgcact gctntacaan aaaattntgg
     1081 aaaaaanttt tgatgtana
//
