LOCUS       sequence-118769-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attached CFP(159-238) to the C-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Unpublished BiFC Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CFP(159-238)Stop in pcDNA1/amp
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     271..292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(387..405)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(388..405)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          489..554
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             684..704
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag cttggtaccg agctcggatc caagaacggc atcaaggccc acttcaagat
      121 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      181 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      241 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      301 cgggatcact ctcggcatgg acgagctgta caagtaaaga tctatcacac tggcggccgc
      361 tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag aggatctttg
      421 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
      481 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      541 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
      601 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
      661 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
      721 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
      781 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
//
