LOCUS       sequence-118770-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attached CFP(159-238) to the C-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     Unpublished BiFC Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CFP(159-238)Stop in pcDNA1/amp
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..75)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(285..304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(286..304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(349..552)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(378..398)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atagatcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
       61 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      121 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      181 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagtgggcc
      241 ttgatgccgt tcttggatcc gagctcggta ccaagcttgg gtctccctat agtgagtcgt
      301 attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat
      361 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      421 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      481 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      541 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      601 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      661 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      721 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
      781 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagcc
//
